Ovariectomy and timing of impaired maxillary alveolar bone regeneration: An experimental study in rats.
X-ray microtomography
bone density
osteoporosis
ovariectomy
Journal
Journal of periodontology
ISSN: 1943-3670
Titre abrégé: J Periodontol
Pays: United States
ID NLM: 8000345
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
18
09
2019
revised:
25
12
2019
accepted:
30
12
2019
pubmed:
22
1
2020
medline:
18
11
2020
entrez:
22
1
2020
Statut:
ppublish
Résumé
The aim of this study was to seek the critical time for impairment of alveolar bone regeneration after ovariectomy (OVX) in rats. A total of 32 female rats were used. Test group rats were divided into a 2M group (n = 8), a 3M group (n = 8) and a 4M group (n = 8) according to the duration from OVX to defect creation. Bilateral OVX was performed in all test groups, and a sham operation was performed in the control group (n = 8). Drill-hole defects (1.5 mm diameter, 2 mm length) were created on both sides of the maxilla. All rats were euthanized 2 and 4 weeks after the surgery. Microcomputed tomographic (micro-CT), histological, and histomorphometric analyses and in vitro experiments were performed. The 4M group showed significantly less new bone formation and a lower bone mineral density than the other groups in the micro-CT analysis. The histomorphometric analysis also revealed that the 4M group showed significantly less new bone formation than the control and 2M groups. The rats in the 4M group showed significantly higher alkaline phosphatase expression levels and a larger number of calcified nodules than rats in the other groups, whereas osteoclastic activity was significantly lower in the 4M group than in the other groups. The critical time for impairment of alveolar bone regeneration was 4 months after OVX in rats.
Sections du résumé
BACKGROUND
The aim of this study was to seek the critical time for impairment of alveolar bone regeneration after ovariectomy (OVX) in rats.
METHODS
A total of 32 female rats were used. Test group rats were divided into a 2M group (n = 8), a 3M group (n = 8) and a 4M group (n = 8) according to the duration from OVX to defect creation. Bilateral OVX was performed in all test groups, and a sham operation was performed in the control group (n = 8). Drill-hole defects (1.5 mm diameter, 2 mm length) were created on both sides of the maxilla. All rats were euthanized 2 and 4 weeks after the surgery. Microcomputed tomographic (micro-CT), histological, and histomorphometric analyses and in vitro experiments were performed.
RESULTS
The 4M group showed significantly less new bone formation and a lower bone mineral density than the other groups in the micro-CT analysis. The histomorphometric analysis also revealed that the 4M group showed significantly less new bone formation than the control and 2M groups. The rats in the 4M group showed significantly higher alkaline phosphatase expression levels and a larger number of calcified nodules than rats in the other groups, whereas osteoclastic activity was significantly lower in the 4M group than in the other groups.
CONCLUSIONS
The critical time for impairment of alveolar bone regeneration was 4 months after OVX in rats.
Identifiants
pubmed: 31961450
doi: 10.1002/JPER.19-0537
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1357-1366Informations de copyright
© 2020 American Academy of Periodontology.
Références
Ji MX, Yu Q. Primary osteoporosis in postmenopausal women. Chronic Dis Transl Med. 2015;1:9-13.
Wronski TJ, Cintron M, Dann LM. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcif Tissue Int. 1988;43:179-183.
Eriksen EF, Hodgson SF, Eastell R, et al. Cancellous bone remodeling in type I (postmenopausal) osteoporosis: quantitative assessment of rates of formation, resorption, and bone loss at tissue and cellular levels. J Bone Miner Res. 1990;5:311-319.
Tanaka M, Ejiri S, Toyooka E, Kohno S, Ozawa H. Effects of ovariectomy on trabecular structures of rat alveolar bone. J Periodontal Res. 2002;37:161-165.
Mohammad AR, Brunsvold M, Bauer R. The strength of association between systemic postmenopausal osteoporosis and periodontal disease. Int J Prosthodont. 1996;9:479-483.
Tezal M, Wactawski-Wende J, Grossi SG, et al. The relationship between bone mineral density and periodontitis in postmenopausal women. J Periodontol. 2000;71:1492-1498.
Shibli JA, Aguiar KC, Melo L, et al. Histologic analysis of human peri-implant bone in type 1 osteoporosis. J Oral Implantol. 2008;34:12-16.
Blomqvist JE, Alberius P, Isaksson S, Linde A, Hansson BG. Factors in implant integration failure after bone grafting: an osteometric and endocrinologic matched analysis. Int J Oral Maxillofac Surg. 1996;25:63-68.
Schliephake H, Neukam FW, Wichmann M. Survival analysis of endosseous implants in bone grafts used for the treatment of severe alveolar ridge atrophy. J Oral Maxillofac Surg. 1997;55:1227-1233. discussion 1233-1224.
Toffler M. Minimally invasive sinus floor elevation procedures for simultaneous and staged implant placement. N Y State Dent J. 2004;70:38-44.
Turner AS. Animal models of osteoporosis-necessity and limitations. Eur Cell Mater. 2001;1:66-81.
El-Shitany NA, Hegazy S, El-Desoky K. Evidences for antiosteoporotic and selective estrogen receptor modulator activity of silymarin compared with ethinylestradiol in ovariectomized rats. Phytomedicine. 2010;17:116-125.
Kalu DN. The ovariectomized rat model of postmenopausal bone loss. Bone Miner. 1991;15:175-191.
French DL, Muir JM, Webber CE. The ovariectomized, mature rat model of postmenopausal osteoporosis: an assessment of the bone sparing effects of curcumin. Phytomedicine. 2008;15:1069-1078.
Byun JS, Lee SS. Effect of soybeans and sword beans on bone metabolism in a rat model of osteoporosis. Ann Nutr Metab. 2010;56:106-112.
Min SK, Kang HK, Jung SY, Jang DH, Min BM. A vitronectin-derived peptide reverses ovariectomy-induced bone loss via regulation of osteoblast and osteoclast differentiation. Cell Death Differ. 2018;25:268-281.
Tao ZS, Lv YX, Cui W, et al. Effect of teriparatide on repair of femoral metaphyseal defect in ovariectomized rats. Z Gerontol Geriatr. 2016;49:423-428.
Kido HW, Bossini PS, Tim CR, et al. Evaluation of the bone healing process in an experimental tibial bone defect model in ovariectomized rats. Aging Clin Exp Res. 2014;26:473-481.
Lasota A, Danowska-Klonowska D. Experimental osteoporosis-different methods of ovariectomy in female white rats. Rocz Akad Med Bialymst. 2004;49(Suppl 1):129-131.
Omi N, Morikawa N, Ezawa I. The effect of voluntary exercise on bone mineral density and skeletal muscles in the rat model at ovariectomized and sham stages. Bone Miner. 1994;24:211-222.
Boraschi-Diaz I, Komarova SV. The protocol for the isolation and cryopreservation of osteoclast precursors from mouse bone marrow and spleen. Cytotechnology. 2016;68:105-114.
Wang JW, Li W, Xu SW, et al. Osteoporosis influences the middle and late periods of fracture healing in a rat osteoporotic model. Chin J Traumatol. 2005;8:111-116.
He YX, Zhang G, Pan XH, et al. Impaired bone healing pattern in mice with ovariectomy-induced osteoporosis: a drill-hole defect model. Bone. 2011;48:1388-1400.
Huang Y. Combined treatment of vitamin K and teriparatide on bone metabolism and biomechanics in rats with osteoporosis. Exp Ther Med. 2018;15:315-319.
Sugie-Oya A, Takakura A, Takao-Kawabata R, et al. Comparison of treatment effects of teriparatide and the bisphosphonate risedronate in an aged, osteopenic, ovariectomized rat model under various clinical conditions. J Bone Miner Metab. 2016;34:303-314.
Legrand E, Chappard D, Pascaretti C, et al. Trabecular bone microarchitecture, bone mineral density, and vertebral fractures in male osteoporosis. J Bone Miner Res. 2000;15:13-19.
Cesar R, Boffa RS, Fachine LT, et al. Evaluation of trabecular microarchitecture of normal osteoporotic and osteopenic human vertebrae. Procedia Engineering. 2013;59:6-15.
Wronski TJ, Walsh CC, Ignaszewski LA. Histologic evidence for osteopenia and increased bone turnover in ovariectomized rats. Bone. 1986;7:119-123.
Iwamoto J, Seki A, Matsuura M, et al. Influence of ovariectomy on bone turnover and trabecular bone mass in mature cynomolgus monkeys. Yonsei Med J. 2009;50:358-367.
Westerlind KC, Wronski TJ, Ritman EL, et al. Estrogen regulates the rate of bone turnover but bone balance in ovariectomized rats is modulated by prevailing mechanical strain. Proc Natl Acad Sci U S A. 1997;94:4199-4204.
Boyce RW, Franks AF, Jankowsky ML, et al. Sequential histomorphometric changes in cancellous bone from ovariohysterectomized dogs. J Bone Miner Res. 1990;5:947-953.
Lai N, Zhang Z, Wang B, et al. Regulatory effect of traditional Chinese medicinal formula Zuo-Gui-Wan on the Th17/Treg paradigm in mice with bone loss induced by estrogen deficiency. J Ethnopharmacol. 2015;166:228-239.
Gallagher JC. The pathogenesis of osteoporosis. Bone Miner. 1990;9:215-227.
Stepan JJ, Pospichal J, Presl J, Pacovsky V. Bone loss and biochemical indices of bone remodeling in surgically induced postmenopausal women. Bone. 1987;8:279-284.
Thompson DD, Simmons HA, Pirie CM, Ke HZ. FDA guidelines and animal models for osteoporosis. Bone. 1995;17:125S-133S.
Walker KV, Kember NF. Cell kinetics of growth cartilage in the rat tibia. II. Measurements during ageing. Cell Tissue Kinet. 1972;5:409-419.
Walker KV, Kember NF. Cell kinetics of growth cartilage in the rat tibia. I. Measurements in young male rats. Cell Tissue Kinet. 1972;5:401-408.