Highly Sensitive Detection of
AML
IDH2 R140Q
IDH2 R172K
PNA-PCR clamping
diagnostic assay
droplet digital PCR
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
19 Jan 2020
19 Jan 2020
Historique:
received:
17
12
2019
revised:
13
01
2020
accepted:
16
01
2020
entrez:
23
1
2020
pubmed:
23
1
2020
medline:
23
1
2020
Statut:
epublish
Résumé
Acute myeloid leukemia is a heterogeneous hematological disease, characterized by karyotypic and molecular alterations. Mutations in Performances of PNA-PCR clamping, droplet digital PCR and Sanger for In contrast with Sanger sequencing, our results highlighted the concordance between PNA clamping and digital PCR. Furthermore, PNA-PCR clamping was able to detect more mutated DNA with respect to Sanger sequencing that showed several false negatives independently from the allelic frequency. We found that PNA-PCR clamping and digital PCR identified
Sections du résumé
BACKGROUND
BACKGROUND
Acute myeloid leukemia is a heterogeneous hematological disease, characterized by karyotypic and molecular alterations. Mutations in
METHODS
METHODS
Performances of PNA-PCR clamping, droplet digital PCR and Sanger for
RESULTS
RESULTS
In contrast with Sanger sequencing, our results highlighted the concordance between PNA clamping and digital PCR. Furthermore, PNA-PCR clamping was able to detect more mutated DNA with respect to Sanger sequencing that showed several false negatives independently from the allelic frequency.
CONCLUSIONS
CONCLUSIONS
We found that PNA-PCR clamping and digital PCR identified
Identifiants
pubmed: 31963812
pii: jcm9010271
doi: 10.3390/jcm9010271
pmc: PMC7019902
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 10005
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