Possible effects of pituitary adenylate cyclase activating polypeptide (PACAP) on early embryo implantation marker HB-EGF in mouse.
Embryo
HB-EGF
Implantation
Mouse
PACAP
Journal
Reproductive biology
ISSN: 2300-732X
Titre abrégé: Reprod Biol
Pays: Poland
ID NLM: 101160559
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
27
02
2019
revised:
10
01
2020
accepted:
15
01
2020
pubmed:
23
1
2020
medline:
31
12
2020
entrez:
23
1
2020
Statut:
ppublish
Résumé
Pituitary adenylate cyclase activating polypeptide (PACAP) was originally isolated as a hypothalamic neuropeptide stimulating adenylate cyclase activity. Besides its neuroprotective effects, numerous data proved its role in reproductive processes. However, there are limited data on its role in preimplantation embryo development and implantation. Our aim was to analyse the mRNA expression of Adcyap1 (coding region of PACAP) and Hbegf [coding region of HB-EGF (Heparin-binding EGF-like growth factor)] in embryos and pregnant uterus to investigate the possible correlation between them. Eight-week-old BDF1 mice were superovulated and subsequently mated overnight or left in their cage after hCG treatment. Day4 embryos were flushed from mated females. After morphological analysis, Adcyap1 and Hbegf gene expression of embryos and uterine tissues was assessed with qPCR. Our results showed significantly higher Adcyap1 and Hbegf mRNA levels in females producing embryos compared to non-mated ones. Robust elevation of Adcyap1 and slight elevation of Hbegf were detected in females with blastocyst embryos compared with non-blastocysts. We found low rate of Hbegf mRNA expression in uncompacted embryos, whereas morulae and blastocysts expressed high amounts of Hbegf. However, we did not find detectable Adcyap1 mRNA in embryos. Strong correlation was found between uterine tissue and embryonic Hbegf levels, slight correlation between uterine Adcyap1 and Hbegf levels. Uterine tissue Adcyap1 and embryonic Hbegf showed no correlation. In summary, our present data show, for the first time, the correlation between PACAP and HB-EGF mRNA expression suggesting that PACAP might play a role during the peri-implantation period of early mouse embryo development.
Identifiants
pubmed: 31964586
pii: S1642-431X(19)30067-1
doi: 10.1016/j.repbio.2020.01.005
pii:
doi:
Substances chimiques
Adcyap1 protein, mouse
0
Heparin-binding EGF-like Growth Factor
0
Pituitary Adenylate Cyclase-Activating Polypeptide
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9-13Informations de copyright
Copyright © 2020 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The Authors declare that there is no conflict of interest.