Kinetic coupling of the respiratory chain with ATP synthase, but not proton gradients, drives ATP production in cristae membranes.
Adenosine Triphosphate
/ biosynthesis
Electron Transport
Hydrogen-Ion Concentration
Kinetics
Mitochondria
/ chemistry
Mitochondrial Membranes
/ chemistry
Mitochondrial Proton-Translocating ATPases
/ genetics
Oxidative Phosphorylation
Proteolipids
/ metabolism
Proton Pumps
/ metabolism
Protons
Saccharomyces cerevisiae
/ genetics
ATP synthesis
cristae
energy conversion
kinetic coupling
mitochondria
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
04 02 2020
04 02 2020
Historique:
pubmed:
23
1
2020
medline:
5
6
2020
entrez:
23
1
2020
Statut:
ppublish
Résumé
Mitochondria have a characteristic ultrastructure with invaginations of the inner membrane called cristae that contain the protein complexes of the oxidative phosphorylation system. How this particular morphology of the respiratory membrane impacts energy conversion is currently unknown. One proposed role of cristae formation is to facilitate the establishment of local proton gradients to fuel ATP synthesis. Here, we determined the local pH values at defined sublocations within mitochondria of respiring yeast cells by fusing a pH-sensitive GFP to proteins residing in different mitochondrial subcompartments. Only a small proton gradient was detected over the inner membrane in wild type or cristae-lacking cells. Conversely, the obtained pH values did barely permit ATP synthesis in a reconstituted system containing purified yeast F
Identifiants
pubmed: 31964824
pii: 1917968117
doi: 10.1073/pnas.1917968117
pmc: PMC7007565
doi:
Substances chimiques
Proteolipids
0
Proton Pumps
0
Protons
0
proteoliposomes
0
Adenosine Triphosphate
8L70Q75FXE
Mitochondrial Proton-Translocating ATPases
EC 3.6.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2412-2421Déclaration de conflit d'intérêts
The authors declare no competing interest.
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