Wide-field time-gated SPAD imager for phasor-based FLIM applications.
Journal
Methods and applications in fluorescence
ISSN: 2050-6120
Titre abrégé: Methods Appl Fluoresc
Pays: England
ID NLM: 101608648
Informations de publication
Date de publication:
05 Feb 2020
05 Feb 2020
Historique:
pubmed:
23
1
2020
medline:
23
1
2020
entrez:
23
1
2020
Statut:
epublish
Résumé
We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512 × 512 pixels), even in the case of small number of gates and limited photon budget.
Identifiants
pubmed: 31968310
doi: 10.1088/2050-6120/ab6ed7
pmc: PMC8827132
mid: NIHMS1772745
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
024002Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM095904
Pays : United States
Références
Biophys Rev. 2011 Jun;3(2):63-70
pubmed: 28510004
Biomed Opt Express. 2016 Apr 11;7(5):1797-814
pubmed: 27231622
Photochem Photobiol Sci. 2005 Jan;4(1):13-22
pubmed: 15616687
Biophys J. 2008 Jan 15;94(2):L14-6
pubmed: 17981902
Light Sci Appl. 2019 Sep 18;8:87
pubmed: 31645931
Methods Appl Fluoresc. 2020 Feb 05;8(2):024002
pubmed: 31968310
Microsc Res Tech. 2008 Mar;71(3):201-13
pubmed: 18008362
Nucl Instrum Methods Phys Res A. 2006 Nov 1;567(1):133
pubmed: 20151021
Biophys J. 2000 Apr;78(4):2127-37
pubmed: 10733990
IEEE J Sel Top Quantum Electron. 2019 Jan-Feb;25(1):
pubmed: 31156324
Opt Express. 2014 Jul 14;22(14):17573-89
pubmed: 25090572
Biomed Opt Express. 2010 Jul 15;1(1):143-156
pubmed: 21258454
J Biophotonics. 2019 Mar;12(3):e201800185
pubmed: 30421551
J Biophotonics. 2014 Aug;7(8):589-96
pubmed: 23576407
Science. 2005 Jan 28;307(5709):538-44
pubmed: 15681376
Chem Rev. 2010 May 12;110(5):2641-84
pubmed: 20356094
Opt Express. 2013 May 20;21(10):11769-82
pubmed: 23736399
J Biomed Opt. 2012 Jan;17(1):016008
pubmed: 22352658
Biophys J. 2002 Dec;83(6):3570-7
pubmed: 12496124
Anal Biochem. 2011 Mar 1;410(1):70-6
pubmed: 21078289