Cutting Edge: The RNA-Binding Protein Ewing Sarcoma Is a Novel Modulator of Lymphotoxin β Receptor Signaling.
Granulocyte-Macrophage Colony-Stimulating Factor
/ genetics
HEK293 Cells
Humans
Lymphotoxin beta Receptor
/ genetics
MAP Kinase Signaling System
/ genetics
Mitogen-Activated Protein Kinase 1
/ genetics
Multiprotein Complexes
/ genetics
RNA-Binding Protein EWS
/ genetics
TNF Receptor-Associated Factor 2
/ genetics
TNF Receptor-Associated Factor 3
/ genetics
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 03 2020
01 03 2020
Historique:
received:
24
10
2019
accepted:
22
12
2019
pubmed:
24
1
2020
medline:
9
9
2020
entrez:
24
1
2020
Statut:
ppublish
Résumé
Lymphotoxin β receptor (LTβR) signaling is crucial for lymphoid tissue organogenesis and immune homeostasis. To identify novel regulatory mechanisms for signaling, we implemented a two-step screen that uses coexpression analysis of human fibroblasts undergoing LTβR stimulation and affinity-purification mass spectrometry for the LTβR signaling protein TNFR-associated factor 3 (TRAF3). We identify Ewing sarcoma (EWS) protein as a novel LTβR signaling component that associates with TRAF3 but not with TNFR-associated factor 2 (TRAF2). The EWS:TRAF3 complex forms under unligated conditions that are disrupted following activation of the LTβR. We conclude that EWS limits expression of proinflammatory molecules, GM-CSF, and ERK-2, promoting immune homeostasis.
Identifiants
pubmed: 31969387
pii: jimmunol.1901260
doi: 10.4049/jimmunol.1901260
pmc: PMC7033016
mid: NIHMS1547578
doi:
Substances chimiques
EWSR1 protein, human
0
LTBR protein, human
0
Lymphotoxin beta Receptor
0
Multiprotein Complexes
0
PSMD2 protein, human
0
RNA-Binding Protein EWS
0
TNF Receptor-Associated Factor 2
0
TNF Receptor-Associated Factor 3
0
TRAF3 protein, human
0
Granulocyte-Macrophage Colony-Stimulating Factor
83869-56-1
MAPK1 protein, human
EC 2.7.11.24
Mitogen-Activated Protein Kinase 1
EC 2.7.11.24
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1085-1090Subventions
Organisme : NIAID NIH HHS
ID : R01 AI067890
Pays : United States
Organisme : NIAMS NIH HHS
ID : T32 AR064194
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA030199
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA177322
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA164679
Pays : United States
Informations de copyright
Copyright © 2020 by The American Association of Immunologists, Inc.
Références
Immunol Rev. 2008 Jun;223:202-20
pubmed: 18613838
J Biol Chem. 2010 Apr 23;285(17):12971-8
pubmed: 20185819
J Immunol. 2015 Sep 1;195(5):2429-41
pubmed: 26209626
J Biol Chem. 2010 May 28;285(22):17148-55
pubmed: 20348096
Bioinformatics. 2007 Jan 15;23(2):257-8
pubmed: 17098774
J Proteomics. 2014 Apr 4;100:37-43
pubmed: 24513533
Cell Signal. 2006 Jul;18(7):994-1005
pubmed: 16242916
Arthritis Rheum. 2004 Jul;50(7):2140-50
pubmed: 15248211
Nucleic Acids Res. 2019 Jan 8;47(D1):D203-D211
pubmed: 30239819
Infect Immun. 2006 Apr;74(4):2121-7
pubmed: 16552041
BMC Bioinformatics. 2008 Dec 29;9:559
pubmed: 19114008
J Rheumatol. 2008 Jun;35(6):960-8
pubmed: 18412315
PLoS One. 2017 Nov 14;12(11):e0187901
pubmed: 29136653
Cell Res. 2011 Jan;21(1):86-102
pubmed: 21102550
Cell Death Differ. 2014 Jan;21(1):136-45
pubmed: 24185621
Mol Syst Biol. 2011 Oct 11;7:536
pubmed: 21988832
Proteins. 2008 Sep;72(4):1125-37
pubmed: 18320585
Sci Signal. 2013 Jan 15;6(258):rs3
pubmed: 23322906
Genome Biol. 2014;15(12):550
pubmed: 25516281
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6244-9
pubmed: 19332782
Ann Lab Med. 2015 Jan;35(1):105-10
pubmed: 25553289
J Biol Chem. 2000 Sep 8;275(36):27858-64
pubmed: 10878013
Am J Respir Crit Care Med. 2000 Apr;161(4 Pt 1):1294-9
pubmed: 10764326
Cold Spring Harb Perspect Biol. 2014 Dec 18;7(4):a016279
pubmed: 25524549
J Immunol. 2010 Aug 1;185(3):1949-58
pubmed: 20592286
Exp Cell Res. 2002 Aug 15;278(2):166-74
pubmed: 12169272
Nat Methods. 2013 Aug;10(8):730-6
pubmed: 23921808
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2460-5
pubmed: 9122217
Nucleic Acids Res. 2017 Dec 1;45(21):12481-12495
pubmed: 30053258
Sarcoma. 2011;2011:837474
pubmed: 21197473
PLoS One. 2014 Nov 18;9(11):e112545
pubmed: 25405351
J Clin Invest. 2007 May;117(5):1314-23
pubmed: 17415412