Middle East Respiratory Syndrome Coronavirus Antibodies in Bactrian and Hybrid Camels from Dubai.


Journal

mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533

Informations de publication

Date de publication:
22 01 2020
Historique:
entrez: 24 1 2020
pubmed: 24 1 2020
medline: 22 9 2020
Statut: epublish

Résumé

So far, dromedary camels are the only known animal reservoir for Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV). Previous published serological studies showed that sera of Bactrian camels were all negative for MERS-CoV antibodies. However, a recent study revealed that direct inoculation of Bactrian camels intranasally with MERS-CoV can lead to infection with abundant virus shedding and seroconversion. In this study, we examined the presence of MERS-CoV antibodies in Bactrian and hybrid camels in Dubai, the United Arab Emirates (where dromedaries are also present), and Bactrian camels in Xinjiang, China (where dromedaries are absent). For the 29 serum samples from Bactrian camels in Dubai tested by the MERS-CoV spike (S) protein-based enzyme-linked immunosorbent assay (S-ELISA) and neutralization antibody test, 14 (48%) and 12 (41%), respectively, were positive for MERS-CoV antibodies. All the 12 serum samples that were positive with the neutralization antibody test were also positive for the S-ELISA. For the 11 sera from hybrid camels in Dubai tested with the S-ELISA and neutralization antibody test, 6 (55%) and 9 (82%), respectively, were positive for MERS-CoV antibodies. All the 6 serum samples that were positive for the S-ELISA were also positive with the neutralization antibody test. There was a strong correlation between the antibody levels detected by S-ELISA and neutralizing antibody titers, with a Spearman coefficient of 0.6262 (

Identifiants

pubmed: 31969478
pii: 5/1/e00898-19
doi: 10.1128/mSphere.00898-19
pmc: PMC6977179
pii:
doi:

Substances chimiques

Antibodies, Viral 0
Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2020 Lau et al.

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Auteurs

Susanna K P Lau (SKP)

State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.
Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong.
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

Kenneth S M Li (KSM)

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

Hayes K H Luk (HKH)

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

Zirong He (Z)

Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

Jade L L Teng (JLL)

State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.
Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

Kwok-Yung Yuen (KY)

State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong.
Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong.
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

Ulrich Wernery (U)

Central Veterinary Research Laboratory, Dubai, United Arab Emirates cvrl@cvrl.ae pcywoo@hku.hk.

Patrick C Y Woo (PCY)

State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong cvrl@cvrl.ae pcywoo@hku.hk.
Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong.
Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong.
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong.

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Classifications MeSH