Copper promotes migration of adipose-derived stem cells by enhancing vimentin-Ser39 phosphorylation.
Copper
Migration
Phospho-vimentin Ser39
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
15 03 2020
15 03 2020
Historique:
received:
17
12
2019
revised:
16
01
2020
accepted:
19
01
2020
pubmed:
24
1
2020
medline:
11
11
2020
entrez:
24
1
2020
Statut:
ppublish
Résumé
Mesenchymal stem cells (MSCs) are widely studied for their application in cell therapy. A noticed drawback of these cells in response to tissue injury is the low efficiency of homing. The present study was undertaken to explore a possible approach to promote the migration of MSCs. Primary cultures of rat adipose-derived stem cells (rADSCs) were cultured in standard L-DMEM media supplemented with or without copper (Cu) at its final concentration of 20 μM in cultures. The analyses of transwell and wound-healing assay revealed that Cu supplementation significantly promotes the migration of rADSCs in cultures. Further analysis found that Cu stimulated the phosphorylation of vimentin Ser39. Point mutation of vimentin Ser39 by substituting Ser with Ala prevented Cu-promoted migration of rADSCs. This study thus demonstrates that Cu promotes migration of rADSCs in cultures through at least in part Cu stimulation of vimentin Ser39 phosphorylation.
Identifiants
pubmed: 31972217
pii: S0014-4827(20)30053-7
doi: 10.1016/j.yexcr.2020.111859
pii:
doi:
Substances chimiques
Vimentin
0
Copper
789U1901C5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111859Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest All authors report no conflicts of interest.