Wound healing properties and antimicrobial activity of platelet-derived biomaterials.
Animals
Anti-Bacterial Agents
/ therapeutic use
Biocompatible Materials
/ therapeutic use
Blood Platelets
/ chemistry
Burns
/ drug therapy
Cell Extracts
/ therapeutic use
Disk Diffusion Antimicrobial Tests
Humans
Male
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Pseudomonas Infections
/ drug therapy
Pseudomonas aeruginosa
/ drug effects
Rats
Rats, Wistar
Staphylococcal Infections
/ drug therapy
Wound Healing
/ drug effects
Wound Infection
/ drug therapy
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 01 2020
23 01 2020
Historique:
received:
12
11
2019
accepted:
06
01
2020
entrez:
25
1
2020
pubmed:
25
1
2020
medline:
11
11
2020
Statut:
epublish
Résumé
We analyzed the potential antibacterial effects of two different PdB against methicillin-resistant S. aureus and P. aeruginosa. The third-degree burn wound healing effects of PdB was also studied. Blood samples were obtained from 10 healthy volunteers and biological assays of the PdB were performed and the antimicrobial activity against MRSA and P. aeruginosa was determined using disk diffusion (DD), broth microdilution (BMD), and time-kill assay methods. 48 Wistar albino rats were burned and infected with MRSA. Two groups were injected PdB, the control groups were treated with plasma and received no treatment respectively. In the next step, the rats were euthanized and skin biopsies were collected and histopathologic changes were examined. The results of DD and BMD showed that both PdB performed very well on MRSA, whereas P. aeruginosa was only inhibited by F-PdB and was less susceptible than MRSA to PdBs. The time-kill assay also showed that F-PdB has an antibacterial effect at 4 hours for two strains. Histopathological studies showed that the treated groups had less inflammatory cells and necrotic tissues. Our data suggest that PdB may possess a clinical utility as a novel topical antimicrobial and wound healing agent for infected burn wounds.
Identifiants
pubmed: 31974417
doi: 10.1038/s41598-020-57559-w
pii: 10.1038/s41598-020-57559-w
pmc: PMC6978467
doi:
Substances chimiques
Anti-Bacterial Agents
0
Biocompatible Materials
0
Cell Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1032Références
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