A prospective pilot study of the T-lymphocyte response to fine particulate matter exposure.
Activation, Metabolic
Adult
Age Factors
Aged
Aged, 80 and over
Air Pollutants
/ toxicity
Cytochrome P-450 CYP1A1
/ genetics
Cytochrome P-450 CYP1B1
/ genetics
Cytochrome P-450 Enzyme System
/ genetics
Cytokines
/ metabolism
Female
Gene Expression Regulation, Enzymologic
Humans
Male
Middle Aged
Oxidative Stress
/ drug effects
Particle Size
Particulate Matter
/ toxicity
Pilot Projects
Prospective Studies
T-Lymphocytes
/ drug effects
Young Adult
T lymphocytes
age
air pollution
fine particulate matter
immunological profile
metabolic activation
Journal
Journal of applied toxicology : JAT
ISSN: 1099-1263
Titre abrégé: J Appl Toxicol
Pays: England
ID NLM: 8109495
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
pubmed:
25
1
2020
medline:
13
7
2021
entrez:
25
1
2020
Statut:
ppublish
Résumé
Exposure to air pollution is associated with increased morbidity and mortality. Once the fine atmospheric particulate matter (FP) is inhaled, some of its compounds can pass through the lungs and reach the bloodstream where they can come into contact with immune cells. Exposure to FP particularly affects sensitive populations such as the elderly. Aging affects the immune system, making the elderly more vulnerable. The project aims to determine the effects of FP exposure on human T cells while looking for biomarkers associated with exposure. Blood samples from 95 healthy subjects in three different age groups (20-30, 45-55 and 70-85 years) were collected to determine a potential age effect. T lymphocytes were isolated to be exposed ex vivo for 72 hours to 45 μg/mL of FP collected in Dunkirk and chemically characterized. Overexpression of the CYP1A1, CYP1B1 and CYP2S1 genes was therefore measured after exposure of the T cells to FP. These genes code for enzymes known to be involved in the metabolic activation of organic compounds such as polycyclic aromatic hydrocarbons detected in the FP sample. T-cell profiling allowed us to suggest a mixed T-helper 1/2 profile caused by exposure to FP. With regard to the influence of age, we have observed differences in the expression of certain genes, as well as an increase in interleukin-4 and -13 concentrations in the elderly. These results showed that exposure of T lymphocytes to FP causes effects on both transcriptomic and cytokine secretion levels.
Substances chimiques
Air Pollutants
0
Cytokines
0
Particulate Matter
0
Cytochrome P-450 Enzyme System
9035-51-2
CYP2S1 protein, human
EC 1.14.-
CYP1A1 protein, human
EC 1.14.14.1
CYP1B1 protein, human
EC 1.14.14.1
Cytochrome P-450 CYP1A1
EC 1.14.14.1
Cytochrome P-450 CYP1B1
EC 1.14.14.1
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
619-630Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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