Development of a polyclonal antibody-based indirect competitive ELISA for the determination of tetrodotoxins in marine ribbon worms (NEMERTEA) and its comparison with high performance liquid chromatography-tandem mass spectrometry.

Animals Aquatic Organisms / chemistry Chromatography, High Pressure Liquid Enzyme-Linked Immunosorbent Assay / methods Tandem Mass Spectrometry / methods Tetrodotoxin / toxicity
Antibodies ELISA Guanidinium toxins HPLC-MS/MS Tetrodotoxin Tetrodotoxin analogues

Journal

Toxicon : official journal of the International Society on Toxinology
ISSN: 1879-3150
Titre abrégé: Toxicon
Pays: England
ID NLM: 1307333

Informations de publication

Date de publication:
Mar 2020
Historique:
received: 14 08 2019
revised: 13 01 2020
accepted: 15 01 2020
pubmed: 25 1 2020
medline: 18 6 2020
entrez: 25 1 2020
Statut: ppublish

Résumé

Tetrodotoxin (TTX) is a potent neurotoxin frequently occurring in marine organisms along with its numerous analogues. To determine the total TTX content, we developed an enzyme-linked immunosorbent assay (ELISA) technique utilizing polyclonal antibodies against TTX. The technique was tested using extracts of marine worms of the phylum Nemertea and confirmed by HPLC-MS/MS. It proved to be suitable for a preliminary assessment of the toxicity of marine organisms.

Identifiants

DOI: 10.1016/j.toxicon.2020.01.009 PMID: 31975690
pubmed: 31975690
pii: S0041-0101(20)30017-9
doi: 10.1016/j.toxicon.2020.01.009
pii:
doi:

Substances chimiques

Tetrodotoxin 4368-28-9

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

30-33

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

A E Vlasenko (AE)

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia.

V G Kuznetsov (VG)

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia; School of Natural Sciences, Far Eastern Federal University, Vladivostok, 690092, Russia.

I Yu Petrova (IY)

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia.

T Yu Magarlamov (TY)

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, 690092, Russia. Electronic address: biotimur@yandex.ru.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Development of a polyclonal antibody-based...
questionsmedicales.fr › Phénomènes biologiques › Phénomènes écologiques et environnementaux › Eaux salées › Eau de mer › Organismes aquatiques › Development of a polyclonal antibody-based...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Development of a polyclonal antibody-based...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Development of a polyclonal antibody-based...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Development of a polyclonal antibody-based...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines de la flore et de la faune marines › Tétrodotoxine › Development of a polyclonal antibody-based...