Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies.
Journal
Evidence-based complementary and alternative medicine : eCAM
ISSN: 1741-427X
Titre abrégé: Evid Based Complement Alternat Med
Pays: United States
ID NLM: 101215021
Informations de publication
Date de publication:
2019
2019
Historique:
received:
11
07
2019
revised:
08
09
2019
accepted:
16
10
2019
entrez:
25
1
2020
pubmed:
25
1
2020
medline:
25
1
2020
Statut:
epublish
Résumé
Wortmannin (WTN) is a steroid metabolite that inhibits phosphatidylinositol 3-kinase and other signaling pathways. Structurally, the WTN consists of a cyclopentanophenanthrene-like structure with several oxygen-rich moieties which have the potential to interact with deoxyribonucleic acid (DNA) molecules. We aim to evaluate the WTN and calf thymus DNA (ct-DNA) interaction with molecular docking using the AutoDock 4.2 software. UV and fluorescence spectroscopy and viscosity techniques were performed to confirm the Molecular docking showed that the WTN interacted with ct-DNA via hydrogen bonds at guanine-rich sequences. The number of hydrogen bonds between the WTN and DNA was 1-2 bonds (average 1.2) per WTN molecule. The These results suggest that the WTN may exert its biological effects, at least in part, via interaction with DNA.
Identifiants
pubmed: 31975999
doi: 10.1155/2019/4936351
pmc: PMC6949734
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4936351Informations de copyright
Copyright © 2019 Shiva Mehran et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interest.
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