Quercetin Decreased Alveolar Bone Loss and Apoptosis in Experimentally Induced Periodontitis Model in Wistar Rats.
Alveolar Bone Loss
/ drug therapy
Animals
Anti-Inflammatory Agents
/ pharmacology
Apoptosis
/ drug effects
Disease Models, Animal
Facial Bones
/ drug effects
Female
Matrix Metalloproteinase 8
/ metabolism
Nitric Oxide Synthase Type II
/ metabolism
Osteoblasts
/ drug effects
Osteoclasts
/ drug effects
Periodontitis
/ drug therapy
Quercetin
/ pharmacology
Rats, Wistar
Tissue Inhibitor of Metalloproteinase-1
/ metabolism
Anti-inflammatory
MMP-8
antioxidants
experimental periodontitis
iNOS
quercetin
Journal
Anti-inflammatory & anti-allergy agents in medicinal chemistry
ISSN: 1875-614X
Titre abrégé: Antiinflamm Antiallergy Agents Med Chem
Pays: United Arab Emirates
ID NLM: 101462262
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
10
2019
revised:
18
12
2019
accepted:
03
01
2020
pubmed:
25
1
2020
medline:
13
7
2021
entrez:
25
1
2020
Statut:
ppublish
Résumé
Quercetin is a flavonoid which has potent anti-inflammatory, antibacterial, and antioxidant effect. Purpose of this study was to evaluate effects of quercetin on alveolar bone loss and histopathological changes in ligature-induced periodontitis in rats. Wistar rats were divided into four experimental groups: non-ligated control (C, n=8) group; periodontitis (P, n=8) group; ligature and low dose quercetin group (75 mg/kg/day quercetin, Q75 group, n=8); ligature and high dose quercetin group (150 mg/kg/day quercetin, Q150 group, n=8). Silk ligatures were placed at gingival margin of lower first molars of mandibular right quadrant. Study duration was 15 days, and animals were sacrificed end of this period. Changes in alveolar bone levels were clinically measured and tissues were immunohistochemically examined, matrix metalloproteinase 8 (MMP 8), inducible nitric oxide synthase (iNOS), tissue inhibitor of metalloproteinase 1 (TIMP 1), Cysteine-aspartic proteases 3 (Caspase 3), and tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and neutrophil counts were also determined. Alveolar bone loss was highest in P group, and differences among P, Q75, and Q150 groups were significant. Both doses of quercetin decreased TRAP+ osteoclast cells and increased osteoblast cells. Inflammation in P group was also higher than those of C, Q75, and Q150 groups indicating anti-inflammatory effect of quercetin. iNOS, MMP-8, and caspase-3 levels were highest, and TIMP-1 expression was lowest in P group; differences were statistically significant. Within limits of this study, it can be suggested that quercetin administration may reduce alveolar bone loss by increasing osteoblastic activity, decreasing osteoclastic activity, apoptosis, and inflammation in an experimental model of periodontitis.
Sections du résumé
BACKGROUND
BACKGROUND
Quercetin is a flavonoid which has potent anti-inflammatory, antibacterial, and antioxidant effect. Purpose of this study was to evaluate effects of quercetin on alveolar bone loss and histopathological changes in ligature-induced periodontitis in rats.
METHODS
METHODS
Wistar rats were divided into four experimental groups: non-ligated control (C, n=8) group; periodontitis (P, n=8) group; ligature and low dose quercetin group (75 mg/kg/day quercetin, Q75 group, n=8); ligature and high dose quercetin group (150 mg/kg/day quercetin, Q150 group, n=8). Silk ligatures were placed at gingival margin of lower first molars of mandibular right quadrant. Study duration was 15 days, and animals were sacrificed end of this period. Changes in alveolar bone levels were clinically measured and tissues were immunohistochemically examined, matrix metalloproteinase 8 (MMP 8), inducible nitric oxide synthase (iNOS), tissue inhibitor of metalloproteinase 1 (TIMP 1), Cysteine-aspartic proteases 3 (Caspase 3), and tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and neutrophil counts were also determined.
RESULTS AND DISCUSSION
CONCLUSIONS
Alveolar bone loss was highest in P group, and differences among P, Q75, and Q150 groups were significant. Both doses of quercetin decreased TRAP+ osteoclast cells and increased osteoblast cells. Inflammation in P group was also higher than those of C, Q75, and Q150 groups indicating anti-inflammatory effect of quercetin. iNOS, MMP-8, and caspase-3 levels were highest, and TIMP-1 expression was lowest in P group; differences were statistically significant.
CONCLUSION
CONCLUSIONS
Within limits of this study, it can be suggested that quercetin administration may reduce alveolar bone loss by increasing osteoblastic activity, decreasing osteoclastic activity, apoptosis, and inflammation in an experimental model of periodontitis.
Identifiants
pubmed: 31976849
pii: AIAAMC-EPUB-103897
doi: 10.2174/1871523019666200124114503
doi:
Substances chimiques
Anti-Inflammatory Agents
0
TIMP1 protein, rat
0
Tissue Inhibitor of Metalloproteinase-1
0
Quercetin
9IKM0I5T1E
Nitric Oxide Synthase Type II
EC 1.14.13.39
Nos2 protein, rat
EC 1.14.13.39
Matrix Metalloproteinase 8
EC 3.4.24.34
Mmp8 protein, rat
EC 3.4.24.34
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
436-448Informations de copyright
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.