HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
08
2019
accepted:
02
01
2020
entrez:
25
1
2020
pubmed:
25
1
2020
medline:
16
4
2020
Statut:
epublish
Résumé
World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log10 copies/mL. Using the 1,000 copies/mL cut-off, the sensitivity of DBS was 97% (95%CI, 91-100%) and specificity was 75% (95%CI, 19-99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.
Identifiants
pubmed: 31978113
doi: 10.1371/journal.pone.0227929
pii: PONE-D-19-22712
pmc: PMC6980635
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0227929Déclaration de conflit d'intérêts
All authors have declared no conflict of interest.
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