Simple Syntheses of New Pegylated Trehalose Derivatives as a Chemical Tool for Potential Evaluation of Cryoprotectant Effects on Cell Membrane.
click-chemistry
cryoprotection
pegylation
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
23 Jan 2020
23 Jan 2020
Historique:
received:
22
12
2019
revised:
15
01
2020
accepted:
19
01
2020
entrez:
26
1
2020
pubmed:
26
1
2020
medline:
28
11
2020
Statut:
epublish
Résumé
In our work, we developed the synthesis of new polyfunctional pegylated trehalose derivatives and evaluated their cryoprotective effect using flow cytometry. We showed that new compounds (modified trehaloses) bound to appropriate extracellular polymeric cryoprotectants could be helpful as a chemical tool for the evaluation of their potential toxic cell membrane influences. Our aim was to form a chemical tool for the evaluation of cryoprotectant cell membrane influences, which are still not easily predicted during the freezing/thawing process. We combined two basic cryoprotectants: polyethyleneglycols (PEGs) and trehalose in the new chemical compounds-pegylated trehalose hybrids. If PEG and trehalose are chemically bound and trehalose is adsorbed on the cell surface PEGs molecules which are, due to the chemical bonding with trehalose, close to the cell surface, can remove the cell surface hydration layer which destabilizes the cell membrane. This was confirmed by the comparison of new material, PEG, trehalose, and their mixture cryoprotective capabilities.
Identifiants
pubmed: 31979348
pii: molecules25030497
doi: 10.3390/molecules25030497
pmc: PMC7038055
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Polyethylene Glycols
3WJQ0SDW1A
Trehalose
B8WCK70T7I
Dimethyl Sulfoxide
YOW8V9698H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : MEYS SAFMAT CZ.02.1.01/0.0/0.0/16_013/0001406, LO1409
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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