Protein complexes including PGRMC1 and actin-associated proteins are disrupted by AG-205.

Actin Cytoskeleton / metabolism Actins / metabolism Cell Line, Tumor Humans Indoles / metabolism Mass Spectrometry Membrane Proteins / antagonists & inhibitors Protein Binding Receptors for Activated C Kinase / metabolism Receptors, Progesterone / antagonists & inhibitors
Actin cytoskeleton Affinity proteomics Cytochrome b5 domain MAPR Membrane trafficking Membrane-associated progesterone receptor

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
26 03 2020
Historique:
received: 13 12 2019
accepted: 20 12 2019
pubmed: 26 1 2020
medline: 8 10 2020
entrez: 26 1 2020
Statut: ppublish

Résumé

PGRMC1 is a protein from the MAPR family with a range of cellular functions. PGRMC1 has been described to play a role in fertility, neuroprotection, steroidogenesis, membrane trafficking and in cancer cell biology. PGRMC1 represents a likely key regulator of cell metabolism and proliferation, as well as a potential target for anti-cancer therapies. To further understand the functions of PGRMC1 and the mechanism of the small molecule inhibitor of PGRMC1, AG-205, proteins differentially bound to PGRMC1 were identified following AG-205 treatment of MIA PaCa-2 cells. Our results suggest that AG-205 influences PGRMC1 interactions with the actin cytoskeleton. The binding of two PGRMC1-associated proteins that support this, RACK1 and alpha-Actinin-1, was reduced following AG-205 treatment. The biology associated with PGRMC1 binding partners identified here merits further investigation.

Identifiants

DOI: 10.1016/j.bbrc.2019.12.108 PMID: 31980178
pubmed: 31980178
pii: S0006-291X(20)30060-7
doi: 10.1016/j.bbrc.2019.12.108
pii:
doi:

Substances chimiques

AG 205 0
Actins 0
Indoles 0
Membrane Proteins 0
PGRMC1 protein, human 0
Receptors for Activated C Kinase 0
Receptors, Progesterone 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

64-69

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflicts of interest.

Auteurs

Sarah L Teakel (SL)

School of Biomedical Sciences, Charles Sturt University, Wagga, Wagga, NSW, 2650, Australia. Electronic address: steakel@csu.edu.au.

Marina Ludescher (M)

Department of Gynecology and Obstetrics, University Women's Hospital of Düsseldorf, Düsseldorf, Germany. Electronic address: Marina.Ludescher@med.uni-duesseldorf.de.

Bashar M Thejer (BM)

School of Biomedical Sciences, Charles Sturt University, Wagga, Wagga, NSW, 2650, Australia; Department of Biology, College of Science, University of Wasit, Wasit, Iraq. Electronic address: bthejer@csu.edu.au.

Gereon Poschmann (G)

Molecular Proteomics Laboratory, Biomedical Research Center (BMFZ), Heinrich-Heine-Universität, Düsseldorf, Medical Faculty, Düsseldorf, Germany. Electronic address: gereon.poschmann@uni-duesseldorf.de.

Jade K Forwood (JK)

School of Biomedical Sciences, Charles Sturt University, Wagga, Wagga, NSW, 2650, Australia. Electronic address: jforwood@csu.edu.au.

Hans Neubauer (H)

Department of Gynecology and Obstetrics, University Women's Hospital of Düsseldorf, Düsseldorf, Germany. Electronic address: Hans.Neubauer@med.uni-duesseldorf.de.

Michael A Cahill (MA)

School of Biomedical Sciences, Charles Sturt University, Wagga, Wagga, NSW, 2650, Australia. Electronic address: mcahill@csu.edu.au.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Actines › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Récepteurs cytoplasmiques et nucléaires › Récepteurs de kinase-C activée › Protein complexes including PGRMC1 and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Récepteurs cytoplasmiques et nucléaires › Récepteurs à la progestérone › Protein complexes including PGRMC1 and...