Improved workflow for mass spectrometry-based metabolomics analysis of the heart.
Cardiac metabolism
GC-MS
Glycogen
Metabolomics
adrenergic receptor
cardiac metabolism
gas chromatography-mass spectrometry (GC-MS)
glycogen
metabolomics
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
28 02 2020
28 02 2020
Historique:
received:
13
09
2019
revised:
17
01
2020
pubmed:
26
1
2020
medline:
22
10
2020
entrez:
26
1
2020
Statut:
ppublish
Résumé
MS-based metabolomics methods are powerful techniques to map the complex and interconnected metabolic pathways of the heart; however, normalization of metabolite abundance to sample input in heart tissues remains a technical challenge. Herein, we describe an improved GC-MS-based metabolomics workflow that uses insoluble protein-derived glutamate for the normalization of metabolites within each sample and includes normalization to protein-derived amino acids to reduce biological variation and detect small metabolic changes. Moreover, glycogen is measured within the metabolomics workflow. We applied this workflow to study heart metabolism by first comparing two different methods of heart removal: the Langendorff heart method (reverse aortic perfusion) and
Identifiants
pubmed: 31980460
pii: S0021-9258(17)49005-3
doi: 10.1074/jbc.RA119.011081
pmc: PMC7049973
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2676-2686Subventions
Organisme : NIA NIH HHS
ID : R01 AG066653
Pays : United States
Organisme : NHLBI NIH HHS
ID : R56 HL131782
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL131782
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA177558
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001998
Pays : United States
Informations de copyright
© 2020 Andres et al.
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