Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 01 2020
24 01 2020
Historique:
received:
18
04
2019
accepted:
18
12
2019
entrez:
26
1
2020
pubmed:
26
1
2020
medline:
9
4
2020
Statut:
epublish
Résumé
Centromeres are defined by a self-propagating chromatin structure based on stable inheritance of CENP-A containing nucleosomes. Here, we present a genetic screen coupled to pulse-chase labeling that allow us to identify proteins selectively involved in deposition of nascent CENP-A or in long-term transmission of chromatin-bound CENP-A. These include factors with known roles in DNA replication, repair, chromatin modification, and transcription, revealing a broad set of chromatin regulators that impact on CENP-A dynamics. We further identify the SUMO-protease SENP6 as a key factor, not only controlling CENP-A stability but virtually the entire centromere and kinetochore. Loss of SENP6 results in hyper-SUMOylation of CENP-C and CENP-I but not CENP-A itself. SENP6 activity is required throughout the cell cycle, suggesting that a dynamic SUMO cycle underlies a continuous surveillance of the centromere complex that in turn ensures stable transmission of CENP-A chromatin.
Identifiants
pubmed: 31980633
doi: 10.1038/s41467-019-14276-x
pii: 10.1038/s41467-019-14276-x
pmc: PMC6981222
doi:
Substances chimiques
Centromere Protein A
0
Chromatin
0
Protein Subunits
0
Cysteine Endopeptidases
EC 3.4.22.-
SENP6 protein, human
EC 3.4.22.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
501Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 210645/Z/18/Z
Pays : United Kingdom
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