Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti.
Antibodies, Protozoan
/ blood
Antibody Specificity
Antigens, Protozoan
/ immunology
Cross-Sectional Studies
Datasets as Topic
Haiti
/ epidemiology
Humans
Immunoglobulin G
/ blood
Immunomagnetic Separation
/ instrumentation
Malaria, Falciparum
/ blood
Plasmodium falciparum
/ immunology
Quality Control
Recombinant Proteins
/ immunology
Reference Standards
Reproducibility of Results
Serologic Tests
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 01 2020
24 01 2020
Historique:
received:
01
11
2019
accepted:
07
01
2020
entrez:
26
1
2020
pubmed:
26
1
2020
medline:
21
11
2020
Statut:
epublish
Résumé
Measuring antimalarial antibodies can estimate transmission in a population. To compare outputs, standardized laboratory testing is required. Here we describe the in-country establishment and quality control (QC) of a multiplex bead assay (MBA) for three sero-surveys in Haiti. Total IgG data against 21 antigens were collected for 32,758 participants. Titration curves of hyperimmune sera were included on assay plates, assay signals underwent 5-parameter regression, and inspection of the median and interquartile range (IQR) for the y-inflection point was used to determine assay precision. The medians and IQRs were similar for Surveys 1 and 2 for most antigens, while the IQRs increased for some antigens in Survey 3. Levey-Jennings charts for selected antigens provided a pass/fail criterion for each assay plate and, of 387 assay plates, 13 (3.4%) were repeated. Individual samples failed if IgG binding to the generic glutathione-S-transferase protein was observed, with 659 (2.0%) samples failing. An additional 455 (1.4%) observations failed due to low bead numbers (<20/analyte). The final dataset included 609,438 anti-malaria IgG data points from 32,099 participants; 96.6% of all potential data points if no QC failures had occurred. The MBA can be deployed with high-throughput data collection and low inter-plate variability while ensuring data quality.
Identifiants
pubmed: 31980693
doi: 10.1038/s41598-020-57876-0
pii: 10.1038/s41598-020-57876-0
pmc: PMC6981173
doi:
Substances chimiques
Antibodies, Protozoan
0
Antigens, Protozoan
0
Immunoglobulin G
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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