Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis.
Animals
Cells, Cultured
Endometriosis
/ enzymology
Endometrium
/ metabolism
Epithelial Cells
/ drug effects
Estrous Cycle
Female
Fibroblasts
/ drug effects
Fibrosis
Gene Expression Regulation, Enzymologic
/ drug effects
Horse Diseases
/ enzymology
Horses
Matrix Metalloproteinases
/ biosynthesis
RNA, Messenger
/ biosynthesis
Tissue Inhibitor of Metalloproteinases
/ biosynthesis
Transforming Growth Factor beta1
/ pharmacology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 01 2020
24 01 2020
Historique:
received:
30
05
2019
accepted:
07
01
2020
entrez:
26
1
2020
pubmed:
26
1
2020
medline:
21
11
2020
Statut:
epublish
Résumé
Equine endometrial fibrosis (endometrosis) is described as a degenerative chronic condition in the uterus. Its characteristic feature is excessive deposition of extracellular matrix (ECM) components around the endometrial glands and stroma. Although matrix metallopeptidases (MMPs) that mediate ECM turnover are important factors in the process of fibrosis, knowledge of their expression and regulation in endometrosis is limited. In other species, one of the important regulators of MMPs and tissue inhibitors of MMPs (TIMPs) is transforming growth factor (TGF)-β1. The goal of this study was to determine (i) endometrial expression of MMPs and TIMPs during endometrosis and (ii) the effect of TGF-β1 on expression of MMPs and TIMPs in equine endometrial fibroblasts and epithelial cells. In the follicular phase of the estrous cycle, MMP-1, -2, -9, and TIMP concentrations were higher during endometrosis than in healthy endometrium (P < 0.05). In the midluteal phase, MMP-3 concentration was lower in severe endometrosis compared to healthy endometrium (P < 0.05). In fibroblasts, TGF-β1 upregulated MMP-1, -9, -13, and TIMP1, but downregulated MMP-3 secretion (P < 0.05). In epithelial cells, TGF-β1 upregulated MMP-1, -9, -13, and TIMP secretion (P < 0.05). Endometrial expression of MMPs and TIMPs is altered during endometrosis. TGF-β1 is a regulator of endometrial ECM remodeling via its effect on MMPs and TIMPs in equine endometrial fibroblasts and epithelial cells.
Identifiants
pubmed: 31980722
doi: 10.1038/s41598-020-58109-0
pii: 10.1038/s41598-020-58109-0
pmc: PMC6981191
doi:
Substances chimiques
RNA, Messenger
0
TGFB1 protein, human
0
Tissue Inhibitor of Metalloproteinases
0
Transforming Growth Factor beta1
0
Matrix Metalloproteinases
EC 3.4.24.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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