Association of PON1-L55M Genetic Variation and Breast Cancer Risk: A Case-Control Trial.


Journal

Asian Pacific journal of cancer prevention : APJCP
ISSN: 2476-762X
Titre abrégé: Asian Pac J Cancer Prev
Pays: Thailand
ID NLM: 101130625

Informations de publication

Date de publication:
01 Jan 2020
Historique:
received: 14 11 2019
entrez: 28 1 2020
pubmed: 28 1 2020
medline: 26 9 2020
Statut: epublish

Résumé

Paraoxonase 1 (PON1), a multifactorial antioxidant enzyme, has a defensive role against oxidative stress, which is believed to contribute to cancer development. This study aimed to investigate the association of PON1-L55M functional polymorphism with breast cancer risk. In the experimental study, blood samples were collected from 150 healthy women controls and 150 breast cancer subjects. The L55M genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism. Our analysis showed that the genotypes distribution is in Hardy-Weinberg equilibrium for both case and control groups. Our data revealed that there are significant associations between PON1-L55M polymorphism and breast cancer risk in homozygote (OR= 2.13, 95%CI= 1.14-4.00, p= 0.018), dominant (OR= 1.72, 95%CI= 1.07-2.76, p= 0.024), and allelic (OR= 1.55, 95%CI= 1.12-2.15, p= 0.008) models. Our results suggest that the PON1-L55M genetic variation could be a genetic risk factor for breast cancer risk and it could be considered as a molecular biomarker for screening of susceptible women.<br />.

Sections du résumé

BACKGROUND BACKGROUND
Paraoxonase 1 (PON1), a multifactorial antioxidant enzyme, has a defensive role against oxidative stress, which is believed to contribute to cancer development. This study aimed to investigate the association of PON1-L55M functional polymorphism with breast cancer risk.
MATERIAL AND METHODS METHODS
In the experimental study, blood samples were collected from 150 healthy women controls and 150 breast cancer subjects. The L55M genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism.
RESULTS RESULTS
Our analysis showed that the genotypes distribution is in Hardy-Weinberg equilibrium for both case and control groups. Our data revealed that there are significant associations between PON1-L55M polymorphism and breast cancer risk in homozygote (OR= 2.13, 95%CI= 1.14-4.00, p= 0.018), dominant (OR= 1.72, 95%CI= 1.07-2.76, p= 0.024), and allelic (OR= 1.55, 95%CI= 1.12-2.15, p= 0.008) models.
CONCLUSIONS CONCLUSIONS
Our results suggest that the PON1-L55M genetic variation could be a genetic risk factor for breast cancer risk and it could be considered as a molecular biomarker for screening of susceptible women.<br />.

Identifiants

pubmed: 31983193
doi: 10.31557/APJCP.2020.21.1.255
pmc: PMC7294023
pii:
doi:

Substances chimiques

Biomarkers, Tumor 0
Aryldialkylphosphatase EC 3.1.8.1
PON1 protein, human EC 3.1.8.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

255-258

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Auteurs

Amir Farmohammadi (A)

Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran.

Ali Momeni (A)

School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Banafshe Bahmani (B)

Department of Pathology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.

Hossein Ghorbani (H)

Pathology Department, Babol University of Medical Sciences, Babol, Iran.

Ramin Ramzanpour (R)

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

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Classifications MeSH