New cellular imaging of oocytes and preimplantation embryos using Lumitein™: Evaluation of oocyte quality and new information on protein dynamics within the perivitelline space during the one-cell oocyte stage in mice.


Journal

The Journal of reproduction and development
ISSN: 1348-4400
Titre abrégé: J Reprod Dev
Pays: Japan
ID NLM: 9438792

Informations de publication

Date de publication:
10 Apr 2020
Historique:
pubmed: 28 1 2020
medline: 3 2 2021
entrez: 28 1 2020
Statut: ppublish

Résumé

The extracellular matrix between the oocyte and zona pellucida (ZP) plays an important role in mammalian fertilization and preserves the specific environment of the perivitelline space (PVS) during the development of a preimplantation embryo after fertilization. In this study, we applied a highly sensitive luminescent protein dye, Lumitein

Identifiants

pubmed: 31983710
doi: 10.1262/jrd.2019-114
pmc: PMC7175381
doi:

Substances chimiques

Luminescent Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

155-161

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Auteurs

Hiroka Okaji (H)

Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.

Kenta Tetsuka (K)

Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.

Ren Watanabe (R)

Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.
Advanced Biotechnology Center, University of Yamanashi, Yamanashi 400-8510, Japan.

Satoshi Kishigami (S)

Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.
Advanced Biotechnology Center, University of Yamanashi, Yamanashi 400-8510, Japan.

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Classifications MeSH