Prophylaxis against renal ischemia-reperfusion injury in canine model: Stem cell approach.
Journal
Indian journal of urology : IJU : journal of the Urological Society of India
ISSN: 0970-1591
Titre abrégé: Indian J Urol
Pays: India
ID NLM: 8510441
Informations de publication
Date de publication:
Historique:
entrez:
28
1
2020
pubmed:
28
1
2020
medline:
28
1
2020
Statut:
ppublish
Résumé
Stem cell therapy at the time of ischemia/reperfusion (I/R) injury has been hypothesized to attenuate the severity of acute kidney injury and to accelerate the regeneration process in lower animal models. Data in higher animal models is limited and discordant. We aimed to explore the reno-protective effects of stem cells on I/R related renal injury in a canine model. Twenty-seven dogs that were treated with bone marrow-derived mesenchymal stem cells (BM-MSCs) were compared with another 27 dogs treated with adipose tissue-derived MSCs (AT-MSCs) following 90 min of warm ischemia to assess IR injury. Each group was divided into three subgroups (nine dogs each), according to the stem cell dose (5, 10, 15 × 10 In Group I, there was a mean reduction of creatinine clearance by 78%, 64%, and 74% at the three used doses, respectively, at 2 weeks. At 3 months, these kidneys regained a mean of 84%, 92%, and 72%, respectively, of its basal function. In Group II, the reduction of clearance was much more modest with mean of 14%, 6%, and 24% respectively at 2 weeks with more intense recovery of renal function by mean of 90%, 100%, and 76%, respectively, at 3 months. Group I had significantly more tubular necrosis and delayed regeneration compared with the Group II. Expressions of pro-inflammatory markers were upregulated in both the groups with a higher and more sustained expression in Group I. Stem cells protected against ischemic reperfusion injury in a canine model. AT-MSCs provided better protection than BM-MSCs.
Identifiants
pubmed: 31983826
doi: 10.4103/iju.IJU_114_19
pii: IJU-36-44
pmc: PMC6961430
doi:
Types de publication
Journal Article
Langues
eng
Pagination
44-49Informations de copyright
Copyright: © 2020 Indian Journal of Urology.
Déclaration de conflit d'intérêts
Conflicts of Interest: There are no conflicts of interest.
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