High-resolution imaging of organic pharmaceutical crystals by transmission electron microscopy and scanning moiré fringes.
Bright-field STEM
High-resolution TEM
Low dose
Organic crystals
Scanning moiré fringes
Journal
Journal of microscopy
ISSN: 1365-2818
Titre abrégé: J Microsc
Pays: England
ID NLM: 0204522
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
02
10
2019
revised:
16
01
2020
accepted:
23
01
2020
pubmed:
28
1
2020
medline:
27
8
2021
entrez:
28
1
2020
Statut:
ppublish
Résumé
Formulation processing of organic crystalline compounds can have a significant effect on drug properties, such as dissolution rate or tablet strength/hardness. Transmission electron microscopy (TEM) has the potential to resolve the atomic lattice of these crystalline compounds and, for example, identify the defect density on a particular crystal face, provided that the sensitivity of these crystals to irradiation by high-energy electrons can be overcome. Here, we acquire high-resolution (HR) lattice images of the compound furosemide using two different methods: low-dose HRTEM and bright-field (BF) scanning TEM (STEM) scanning moiré fringes (SMFs). Before acquiring HRTEM images of furosemide, a model system of crocidolite (asbestos) was used to determine the electron flux/fluence limits of low-dose HR imaging for our scintillator-based, complementary metal-oxide semiconductor (CMOS) electron camera by testing a variety of electron flux and total electron fluence regimes. An electron flux of 10 e
Identifiants
pubmed: 31985063
doi: 10.1111/jmi.12866
pmc: PMC8611800
doi:
Substances chimiques
Pharmaceutical Preparations
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
197-206Informations de copyright
© 2020 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.
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