Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
15
09
2019
accepted:
13
01
2020
revised:
06
02
2020
pubmed:
28
1
2020
medline:
25
4
2020
entrez:
28
1
2020
Statut:
epublish
Résumé
Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.
Identifiants
pubmed: 31986129
doi: 10.1371/journal.pbio.3000567
pii: PBIOLOGY-D-19-02718
pmc: PMC7004386
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000567Subventions
Organisme : NIGMS NIH HHS
ID : F31 GM123736
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM116000
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Organisme : NIH HHS
ID : S10 OD010580
Pays : United States
Organisme : NIH HHS
ID : DP5 OD017851
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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