Platform with biomimetic electrochemical sensors for adiponectin and leptin detection in human serum.


Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Apr 2020
Historique:
received: 22 07 2019
revised: 07 12 2019
accepted: 10 12 2019
entrez: 29 1 2020
pubmed: 29 1 2020
medline: 23 9 2020
Statut: ppublish

Résumé

Two new biomimetic sensors for the detection of adiponectin (A) and leptin (L) through molecularly imprinted polymers (MIPs) onto gold working electrodes (GWEs) were fabricated. Based on electrochemical impedance spectroscopy (EIS) results and cyclic voltammetry (CV) characteristics recorded in the development stages of the fabricated sensors, the sensors were electrochemically optimized and used in an integrated microfluidic platform to detect adiponectin/leptin via conductance signals and non-imprinted electrodes were used as references. To overcome the limitation of the low response signals after template binding non-conductive polyphenol (PP) and poliscopoletin (PS) were used for templates formation. Under optimized experimental conditions the conductance and resistance signals were obtained in the linear range of 0-50 μg ml

Identifiants

pubmed: 31987187
pii: S0039-9140(19)31276-7
doi: 10.1016/j.talanta.2019.120643
pii:
doi:

Substances chimiques

Adiponectin 0
Biocompatible Materials 0
Leptin 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

120643

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Carmen Marinela Mihailescu (CM)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania; Titu Maiorescu University, Pharmaceutical University, Sincai Street, 040314, Bucharest, Romania. Electronic address: carmen.mihailescu@imt.ro.

Dana Stan (D)

DDS Diagnostic, Vulcan Judetu Street, Bucharest, 031427, Romania.

Mihaela Savin (M)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania; Faculty of Applied Chemistry and Materials Science, University Politehnica Bucharest, 1-7 Gheorghe Polizu St., sector 1, 011061, Bucharest, Romania.

Carmen Aura Moldovan (CA)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

Silviu Dinulescu (S)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

Clara Hortensia Radulescu (CH)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

Bogdan Firtat (B)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

George Muscalu (G)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

Costin Brasoveanu (C)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

Marian Ion (M)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania.

David Dragomir (D)

National Institute for Research and Development in Microtechnologies, Erou Iancu Nicolae Street, Bucharest, 077190, Romania; University Politehnica of Bucharest, Splaiul Independentei Street, no. 313, Bucharest, 060042, Romania.

Diana Stan (D)

DDS Diagnostic, Vulcan Judetu Street, Bucharest, 031427, Romania.

Alina Catrinel Ion (AC)

Faculty of Applied Chemistry and Materials Science, University Politehnica Bucharest, 1-7 Gheorghe Polizu St., sector 1, 011061, Bucharest, Romania.

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Classifications MeSH