Interactions between PAMAM dendrimers and DOPC lipid multilayers: Membrane thinning and structural disorder.

Dendrimers / chemistry Hydrophobic and Hydrophilic Interactions Lipid Bilayers / chemistry Liposomes / chemistry Phosphatidylcholines / chemistry
Lipid multilayers Model membranes Nanoparticle-membrane hybrids Nanoparticle-membrane interactions Nanotoxicity PAMAM dendrimers X-ray reflectivity

Journal

Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726

Informations de publication

Date de publication:
04 2021
Historique:
received: 07 11 2019
revised: 18 01 2020
accepted: 22 01 2020
pubmed: 29 1 2020
medline: 29 6 2021
entrez: 29 1 2020
Statut: ppublish

Résumé

Understanding the structure of hybrid nanoparticle-lipid multilayers is of fundamental importance to their bioanalytical applications and nanotoxicity, where nanoparticle-membrane interactions play an important role. Poly(amidoamine) (PAMAM) dendrimers are branched polymeric nanoparticles with potential biomedical applications due to precise tunability of their physicochemical properties. Here, the effect of PAMAM dendrimers (2.9-4.5 nm) with either a hydrophilic amine (NH DOPC multilayers were created by the liposome-rupture method via drop-casting dendrimer-liposome dispersions with the dendrimers added at different concentrations and at three different stages. The multilayer structure was evaluated via the analysis of the synchrotron X-ray reflectivity (XRR) curves, obtaining the bilayer d-spacing, the coherence length from the Scherrer (L Dendrimer addition led to lipid bilayer thinning and more disordered multilayer structures. Larger hydrophobic dendrimers caused greater structural disruption to the multilayers compared to the smaller dendrimers. The smallest, positively charged dendrimers at their highest concentration caused the most pronounced bilayer thinning. The dendrimer-liposome mixing method also affected the multilayer structure due to different dendrimer aggregation involved. These results show the complexity of the effect of dendrimer physicochemical properties and the addition method of dendrimers on the structure of mixed dendrimer-DOPC multilayers. These insights are useful for fundamental understanding of nanotoxicity and future biomedical application of nanocomposite multilayer materials in which nanoparticles are added for enhanced properties and functionality.

Sections du résumé

BACKGROUND
Understanding the structure of hybrid nanoparticle-lipid multilayers is of fundamental importance to their bioanalytical applications and nanotoxicity, where nanoparticle-membrane interactions play an important role. Poly(amidoamine) (PAMAM) dendrimers are branched polymeric nanoparticles with potential biomedical applications due to precise tunability of their physicochemical properties. Here, the effect of PAMAM dendrimers (2.9-4.5 nm) with either a hydrophilic amine (NH
METHODS
DOPC multilayers were created by the liposome-rupture method via drop-casting dendrimer-liposome dispersions with the dendrimers added at different concentrations and at three different stages. The multilayer structure was evaluated via the analysis of the synchrotron X-ray reflectivity (XRR) curves, obtaining the bilayer d-spacing, the coherence length from the Scherrer (L
RESULTS
Dendrimer addition led to lipid bilayer thinning and more disordered multilayer structures. Larger hydrophobic dendrimers caused greater structural disruption to the multilayers compared to the smaller dendrimers. The smallest, positively charged dendrimers at their highest concentration caused the most pronounced bilayer thinning. The dendrimer-liposome mixing method also affected the multilayer structure due to different dendrimer aggregation involved.
CONCLUSIONS
These results show the complexity of the effect of dendrimer physicochemical properties and the addition method of dendrimers on the structure of mixed dendrimer-DOPC multilayers.
GENERAL SIGNIFICANCE
These insights are useful for fundamental understanding of nanotoxicity and future biomedical application of nanocomposite multilayer materials in which nanoparticles are added for enhanced properties and functionality.

Identifiants

DOI: 10.1016/j.bbagen.2020.129542 PMID: 31987955
pubmed: 31987955
pii: S0304-4165(20)30032-5
doi: 10.1016/j.bbagen.2020.129542
pii:
doi:

Substances chimiques

Dendrimers 0
Lipid Bilayers 0
Liposomes 0
PAMAM Starburst 0
Phosphatidylcholines 0
1,2-oleoylphosphatidylcholine EDS2L3ODLV

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

129542

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Laura J Fox (LJ)

Bristol Centre for Functional Nanomaterials, H. H. Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol BS8 1TL, UK; School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

Anna Slastanova (A)

School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

Nicolas Taylor (N)

School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

Magdalena Wlodek (M)

Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, PL-30239 Krakow, Poland.

Oier Bikondoa (O)

XMaS, The UK-CRG Beamline, The European Synchrotron (ESRF), 71 Avenue des Martyrs, 38043 Grenoble, France; Department of Physics, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.

Robert M Richardson (RM)

School of Physics, H. H. Wills Physics Laboratory, University of Bristol, Tyndall Avenue, Bristol BS8 1TL, UK.

Wuge H Briscoe (WH)

School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK. Electronic address: wuge.briscoe@bristol.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Dendrimères › Interactions between PAMAM dendrimers and DOPC...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Interactions between PAMAM dendrimers and DOPC...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Double couche lipidique › Interactions between PAMAM dendrimers and DOPC...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › Interactions between PAMAM dendrimers and DOPC...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Glycérophosphate › Acides phosphatidiques › Glycérophospholipides › Phosphatidylcholines › Interactions between PAMAM dendrimers and DOPC...