Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.
Hedgehog
Helioxanthin
Mesoderm
Three-dimensional culture
Wnt
Journal
Regenerative therapy
ISSN: 2352-3204
Titre abrégé: Regen Ther
Pays: Netherlands
ID NLM: 101709085
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
05
09
2019
revised:
20
11
2019
accepted:
24
12
2019
entrez:
29
1
2020
pubmed:
29
1
2020
medline:
29
1
2020
Statut:
epublish
Résumé
Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages. Here we developed a fully defined xeno-free strategy to direct hiPSCs toward osteoblasts within 21 days. The strategy successfully achieved the osteogenic induction of four independently derived hiPSC lines by a sequential use of combinations of small-molecule inducers. The induction first generated mesodermal cells, which subsequently recapitulated the developmental expression pattern of major osteoblast genes and proteins. Importantly, Col2.3-Cherry hiPSCs subjected to this strategy strongly expressed the cherry fluorescence that has been observed in bone-forming osteoblasts
Identifiants
pubmed: 31988991
doi: 10.1016/j.reth.2019.12.010
pii: S2352-3204(19)30168-3
pmc: PMC6965656
doi:
Types de publication
Journal Article
Langues
eng
Pagination
19-31Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR052374
Pays : United States
Informations de copyright
© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that they have no conflict of interest.
Références
Development. 2004 Mar;131(6):1309-18
pubmed: 14973297
Nat Methods. 2011 May;8(5):424-9
pubmed: 21478862
Stem Cells Transl Med. 2013 Apr;2(4):255-64
pubmed: 23512959
Stem Cells. 2018 Mar;36(3):349-362
pubmed: 29193426
Stem Cell Res. 2015 Jul;15(1):75-87
pubmed: 26002631
Genes Dev. 1999 Aug 15;13(16):2072-86
pubmed: 10465785
J Dev Biol. 2016 Jun 03;4(2):null
pubmed: 29615586
Development. 2008 Sep;135(17):2969-79
pubmed: 18667462
Dev Cell. 2005 May;8(5):727-38
pubmed: 15866163
Gene. 2003 Oct 16;316:23-32
pubmed: 14563548
Cell Stem Cell. 2014 Jan 2;14(1):13-26
pubmed: 24388173
J Biol Chem. 2008 Feb 15;283(7):3816-26
pubmed: 18056716
PLoS One. 2013;8(1):e55134
pubmed: 23383082
Sci Adv. 2017 May 12;3(5):e1602875
pubmed: 28508073
Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16806-11
pubmed: 17077151
Biomol Eng. 2007 Dec;24(6):613-8
pubmed: 17936069
J Oral Sci. 2013 Mar;55(1):63-70
pubmed: 23485603
PLoS Genet. 2006 Dec;2(12):e216
pubmed: 17194222
Proteomics. 2010 May;10(9):1886-90
pubmed: 20162561
Mol Ther. 2009 Nov;17(11):1967-78
pubmed: 19603005
J Bone Miner Res. 2002 Jan;17(1):15-25
pubmed: 11771662
J Biol Chem. 1993 Mar 15;268(8):5588-93
pubmed: 8449921
Stem Cells Transl Med. 2014 Oct;3(10):1125-37
pubmed: 25122686
Nat Mater. 2016 Mar;15(3):344-52
pubmed: 26752655
Stem Cells Transl Med. 2013 Jan;2(1):16-24
pubmed: 23283489
FASEB J. 2007 Jun;21(8):1777-87
pubmed: 17317722
Development. 2006 Aug;133(16):3231-44
pubmed: 16854976
BMC Genomics. 2003 Nov 07;4:43
pubmed: 14604442
J Biol Chem. 2008 Oct 24;283(43):29119-25
pubmed: 18703512
J Tissue Eng Regen Med. 2018 Feb;12(2):437-446
pubmed: 28494141
Biochem Biophys Res Commun. 2006 Jan 13;339(2):490-8
pubmed: 16298332
Development. 2015 Mar 1;142(5):817-31
pubmed: 25715393
J Biol Chem. 1989 Nov 25;264(33):19934-41
pubmed: 2479640
Eur Cell Mater. 2014 Oct 23;28:269-86
pubmed: 25340806
Dev Cell. 2008 May;14(5):689-99
pubmed: 18477452
PLoS One. 2012;7(8):e42855
pubmed: 22912751
Sci Rep. 2014 Oct 22;4:6716
pubmed: 25336194
Dev Cell. 2005 May;8(5):739-50
pubmed: 15866164
Sci Adv. 2016 Aug 31;2(8):e1600691
pubmed: 27602403
Sci Rep. 2016 May 26;6:26761
pubmed: 27225733
Stem Cells. 2011 Feb;29(2):206-16
pubmed: 21732479
J Bone Miner Res. 2001 Jul;16(7):1228-36
pubmed: 11450698
Cell Mol Life Sci. 2010 Nov;67(22):3837-47
pubmed: 20668908
Stem Cell Reports. 2014 May 22;2(6):751-60
pubmed: 24936463
Cell. 2002 Jan 11;108(1):17-29
pubmed: 11792318
J Biol Chem. 2006 Mar 10;281(10):6297-306
pubmed: 16410254
Methods Mol Biol. 2011;695:281-308
pubmed: 21042979
Stem Cells Transl Med. 2014 Jul;3(7):867-78
pubmed: 24855277
Cell. 2007 Nov 30;131(5):861-72
pubmed: 18035408
Cell. 1997 May 30;89(5):755-64
pubmed: 9182763
PLoS One. 2012;7(10):e47078
pubmed: 23115636
Stem Cell Res. 2015 Jul;15(1):122-129
pubmed: 26042795
Development. 2009 Dec;136(24):4099-110
pubmed: 19906845
J Bone Miner Res. 2014 Sep;29(9):1960-9
pubmed: 24692107
Development. 2005 Jan;132(1):49-60
pubmed: 15576404
Biomaterials. 2013 Jul;34(22):5530-7
pubmed: 23623228
Histochem Cell Biol. 2014 Aug;142(2):217-26
pubmed: 24477550
Biochem Biophys Res Commun. 2007 Jun 15;357(4):854-60
pubmed: 17451649
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13742-7
pubmed: 20643952