Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets.
ADSC, adipose-derived stem cell
APC, allophycocyanin
Adipose-derived stem cell
Anastomotic healing
BAS, biliary anastomotic strictures
BMSC, bone marrow stem cells
Biliary anastomosis
CBD, common bile duct
Cell sheet
FBS, fetal bovine serum
FGF, fibroblast growth factor
HGF, hepatocyte growth factor
MSC, mesenchymal stem cell
VEGF, vascular endothelial growth factor
Journal
Regenerative therapy
ISSN: 2352-3204
Titre abrégé: Regen Ther
Pays: Netherlands
ID NLM: 101709085
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
11
06
2019
revised:
10
10
2019
accepted:
12
11
2019
entrez:
29
1
2020
pubmed:
29
1
2020
medline:
29
1
2020
Statut:
epublish
Résumé
Cell sheets consisting of adipose-derived stem cells (ADSCs) have been reported to be effective for wound healing. We conducted this study to clarify the efficacy of ADSC sheets in wound healing at the duct-to-duct biliary anastomotic site in pigs. Eleven female pigs (20-25 kg) were divided into two groups: biliary anastomosis with an ADSC sheet (n = 6) or without an ADSC sheet (n = 5). To follow the transplanted ADSCs, PKH26GL-labeled sheets were used in one of the ADSC pigs. Two weeks prior to laparotomy, ADSCs were isolated from the lower abdominal subcutaneous adipose tissue. After three passages, ADSCs were seeded on temperature-responsive culture dishes and collected as cell sheets. ADSC sheets were gently transplanted on the anastomotic site. We evaluated specimens by PKH26GL labeling, macroscopic changes, infiltration of inflammatory cells, and collagen content. Labeled ADSCs remained around the bile duct wall. In the no-ADSC group, more adhesion developed at the hepatic hilum as observed during relaparotomy. Histopathological examination showed that the diameter and cross-sectional area of the bile duct wall were decreased in the ADSC group. In the no-ADSC group, a large number of inflammatory cells and more collagen fibers were identified in the bile duct wall. The present study demonstrated that autologous ADSC sheet transplantation reduced hypertrophic changes in the bile duct wall at the anastomotic site. A long-term follow-up is required to evaluate the efficacy of this mechanism in prevention of biliary anastomotic strictures.
Identifiants
pubmed: 31988997
doi: 10.1016/j.reth.2019.11.001
pii: S2352-3204(19)30142-7
pmc: PMC6970135
doi:
Types de publication
Journal Article
Langues
eng
Pagination
79-86Informations de copyright
© 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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