Overexpression of CFL1 in gastric cancer and the effects of its silencing by siRNA with a nanoparticle delivery system in the gastric cancer cell line.
Adenocarcinoma
/ drug therapy
Apoptosis
/ drug effects
Biomarkers, Tumor
/ genetics
Cell Line, Tumor
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Cofilin 1
/ genetics
Drug Delivery Systems
/ methods
Gene Expression Regulation, Neoplastic
/ drug effects
Gene Silencing
/ physiology
Humans
Nanoparticles
/ administration & dosage
RNA, Small Interfering
/ genetics
Stomach Neoplasms
/ drug therapy
Transfection
/ methods
CFL1
gastric cancer
nanoparticle
siRNA
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
26
01
2019
accepted:
13
01
2020
pubmed:
29
1
2020
medline:
9
3
2021
entrez:
29
1
2020
Statut:
ppublish
Résumé
Gastric adenocarcinoma, like other cancers, is a multifactorial genetic disease, and metastasis of cancer cells is one of the main features of this illness. The expression levels of the CFL1 gene have been modulated in this pathway. Using small interfering RNA (siRNA) in the treatment of gastric cancer is considered a hopeful gene therapeutic approach. The present study reported the level of CFL1 genes between tumor and margin and healthy tissue of gastric cancer. Also, the features of a cationic nanoparticle with a polymer coating containing polyacrylic acid and polyethyleneimine that were used in the delivery of CFL1 siRNA, were shown. Then the cytotoxicity, cellular uptake, and gene silencing efficiency of this nanoparticle were evaluated with CFL1siRNA. In this study, the CFL1 gene expression was measured in 40 gastric adenocarcinoma, marginal and 15 healthy biopsy samples by a real-time polymerase chain reaction. Physicochemical characteristics, apoptosis, and inhibition of migration of the delivery of CFL1 siRNA by nanoparticle and lipofectamine were investigated in gastric cancer cells. The CFL1 expression was remarkably increased in gastric cancer tissues in comparison with the marginal samples and normal tissues (p < .05) and the biomarker index for CFL1 was obtained as 0.94, then this gene can be probably used as a biomarker for gastric cancer. After treatment of the AGS cell line by CFL1 siRNA, the CFL1 expression level of mRNA and migration in AGS cells were remarkably suppressed after transfection. Furthermore, the amount of apoptosis increased (p < .05). Our results demonstrated that CFL1 downregulation in AGS cells can interdict cell migration. Finally, our outcomes propose that CFL1 can function as an oncogenic gene in gastric cancer and would be considered as a potential purpose of gene therapy for gastric cancer treatment.
Substances chimiques
Biomarkers, Tumor
0
CFL1 protein, human
0
Cofilin 1
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6660-6672Informations de copyright
© 2020 Wiley Periodicals, Inc.
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