Insight into the resilience and susceptibility of marine bacteria to T6SS attack by Vibrio cholerae and Vibrio coralliilyticus.
Animals
Anthozoa
/ microbiology
Antibodies, Bacterial
/ genetics
Bacterial Proteins
/ genetics
Bacteroidetes
/ genetics
Gene Expression Regulation, Bacterial
/ genetics
Proteobacteria
/ genetics
Shellfish
/ microbiology
Type VI Secretion Systems
/ genetics
Vibrio
/ genetics
Vibrio cholerae
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
21
06
2019
accepted:
31
12
2019
entrez:
29
1
2020
pubmed:
29
1
2020
medline:
17
4
2020
Statut:
epublish
Résumé
The type VI secretion system (T6SS) is a nanomachine capable of killing adjacent microbial cells in a contact-dependent manner. Due to limited studies, relatively little is known about the range of marine bacteria that are susceptible to T6SS attack. Here, 15 diverse marine bacterial isolates from the phyla Bacteroidetes and Ɣ-Proteobacteria were challenged against the marine bacterium and human pathogen, Vibrio cholerae, which has a well described T6SS. V. cholerae killed several of the tested Ɣ-Proteobacteria, including members of the orders Vibrionales, Alteromonadales, Oceanospirillales, and Pseudomonadales. In contrast, V. cholerae co-existed with multiple Bacteroidetes and Ɣ-Proteobacteria isolates, but was killed by Vibrio coralliilyticus. Follow-up experiments revealed that five V. coralliilyticus strains, including known coral and shellfish pathogens survived the T6SS challenge and killed V. cholerae. By using predicted protein comparisons and mutagenesis, we conclude that V. coralliilyticus protected itself in the challenge by using its own T6SS to kill V. cholerae. This study provides valuable insight into the resilience and susceptibility of marine bacteria to the V. cholerae T6SS, and provides the first evidence for a functional T6SS in V. coralliilyticus, both of which have implications for human and ocean health.
Identifiants
pubmed: 31990915
doi: 10.1371/journal.pone.0227864
pii: PONE-D-19-17649
pmc: PMC6986712
doi:
Substances chimiques
Antibodies, Bacterial
0
Bacterial Proteins
0
Type VI Secretion Systems
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0227864Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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