Osteogenic Potential of Bovine Bone Graft in Combination with Laser Photobiomodulation: An Ex Vivo Demonstrative Study in Wistar Rats by Cross-Linked Studies Based on Synchrotron Microtomography and Histology.
Photobiomodulation
bone regeneration
collage membrane
histology
synchrotron radiation-based X-ray microtomography
xenograft
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
25 Jan 2020
25 Jan 2020
Historique:
received:
21
12
2019
revised:
21
01
2020
accepted:
23
01
2020
entrez:
30
1
2020
pubmed:
30
1
2020
medline:
6
11
2020
Statut:
epublish
Résumé
Alveolar bone defects are usually the main concern when planning implant treatments for the appropriate oral rehabilitation of patients. To improve local conditions and achieve implant treatments, there are several methods used for increasing bone volume, among which one of the most successful, versatile, and effective is considered to be guided bone regeneration. The aim of this demonstrative study was to propose an innovative analysis protocol for the evaluation of the effect of photobiomodulation on the bone regeneration process, using rat calvarial defects of 5 mm in diameter, filled with xenograft, covered with collagen membrane, and then exposed to laser radiation. The animals were sacrificed at different points in time (i.e., after 14, 21, and 30 days). Samples of identical dimensions were harvested in order to compare the results obtained after different periods of healing. The analysis was performed by cross-linking the information obtained using histology and high-resolution synchrotron-based tomography on the same samples. A comparison was made with both the negative control (NC) group (with a bone defect which was left for spontaneous healing), and the positive control (PC) group (in which the bone defects were filled with xenografts and collagen membrane without receiving laser treatment). We demonstrated that using photobiomodulation provides a better healing effect than when receiving only the support of the biomaterial. This effect has been evident for short times treatments, i.e., during the first 14 days after surgery. The proposed analysis protocol was effective in detecting the presence of higher quantities of bone volumes under remodeling after photobiomodulation with respect to the exclusive bone regeneration guided by the xenograft.
Sections du résumé
BACKGROUND
BACKGROUND
Alveolar bone defects are usually the main concern when planning implant treatments for the appropriate oral rehabilitation of patients. To improve local conditions and achieve implant treatments, there are several methods used for increasing bone volume, among which one of the most successful, versatile, and effective is considered to be guided bone regeneration. The aim of this demonstrative study was to propose an innovative analysis protocol for the evaluation of the effect of photobiomodulation on the bone regeneration process, using rat calvarial defects of 5 mm in diameter, filled with xenograft, covered with collagen membrane, and then exposed to laser radiation.
METHODS
METHODS
The animals were sacrificed at different points in time (i.e., after 14, 21, and 30 days). Samples of identical dimensions were harvested in order to compare the results obtained after different periods of healing. The analysis was performed by cross-linking the information obtained using histology and high-resolution synchrotron-based tomography on the same samples. A comparison was made with both the negative control (NC) group (with a bone defect which was left for spontaneous healing), and the positive control (PC) group (in which the bone defects were filled with xenografts and collagen membrane without receiving laser treatment).
RESULTS
RESULTS
We demonstrated that using photobiomodulation provides a better healing effect than when receiving only the support of the biomaterial. This effect has been evident for short times treatments, i.e., during the first 14 days after surgery.
CONCLUSION
CONCLUSIONS
The proposed analysis protocol was effective in detecting the presence of higher quantities of bone volumes under remodeling after photobiomodulation with respect to the exclusive bone regeneration guided by the xenograft.
Identifiants
pubmed: 31991756
pii: ijms21030778
doi: 10.3390/ijms21030778
pmc: PMC7037661
pii:
doi:
Substances chimiques
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Romanian National Authority for Scientific Research
ID : PN-III-P2-2.1-PTE-2016-0181
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Photomed Laser Surg. 2006 Apr;24(2):169-78
pubmed: 16706695
Photomed Laser Surg. 2012 Mar;30(3):149-54
pubmed: 22235971
Periodontol 2000. 2015 Jun;68(1):55-65
pubmed: 25867979
J Clin Laser Med Surg. 2003 Dec;21(6):383-8
pubmed: 14709224
Postepy Hig Med Dosw (Online). 2008 Jun 11;62:282-8
pubmed: 18583951
Rev Bras Fisioter. 2011 May-Jun;15(3):200-5
pubmed: 21829983
J Clin Laser Med Surg. 2002 Apr;20(2):83-7
pubmed: 12017432
J Prosthodont Res. 2013 Jan;57(1):3-14
pubmed: 23347794
Oral Oncol. 2018 Jul;82:200-202
pubmed: 29752002
Int J Oral Maxillofac Implants. 2007;22 Suppl:49-70
pubmed: 18437791
Int J Periodontics Restorative Dent. 2013 Jul-Aug;33(4):437-45
pubmed: 23820701
J Biomed Opt. 2014;19(7):071412
pubmed: 25079820
Adv Struct Chem Imaging. 2017;3(1):4
pubmed: 28261542
J Dent (Tehran). 2015 Jan;12(1):31-8
pubmed: 26005452
Open Dent J. 2011;5:96-104
pubmed: 21760862
J Periodontol. 2000 Mar;71(3):488-96
pubmed: 10776939
Dent Mater. 2014 Apr;30(4):417-23
pubmed: 24530139
Bioact Mater. 2017 Jun 07;2(4):224-247
pubmed: 29744432
Photomed Laser Surg. 2005 Apr;23(2):161-6
pubmed: 15910179
J Prosthodont Res. 2018 Apr;62(2):152-161
pubmed: 28927994
Lasers Med Sci. 2014 Sep;29(5):1539-50
pubmed: 23525868
Materials (Basel). 2018 May 02;11(5):
pubmed: 29724045
J Periodontal Res. 2016 Feb;51(1):112-24
pubmed: 26094874
Photomed Laser Surg. 2006 Feb;24(1):38-44
pubmed: 16503787
J Clin Laser Med Surg. 1998 Dec;16(6):317-20
pubmed: 10204437
Curr Pharm Biotechnol. 2017;18(1):33-44
pubmed: 27915982
Lasers Surg Med. 2004;34(3):277-84
pubmed: 15022258
Clin Oral Implants Res. 2000 Dec;11(6):540-5
pubmed: 11168247
Clin Oral Implants Res. 1990 Dec;1(1):22-32
pubmed: 2099209
Photomed Laser Surg. 2007 Aug;25(4):275-80
pubmed: 17803384
J Bone Joint Surg Am. 1963 Sep;45:1305-13
pubmed: 14078540
Bone. 2008 Mar;42(3):456-66
pubmed: 18096457
Braz Dent J. 2014 Sep-Oct;25(5):379-84
pubmed: 25517771
Int J Periodontics Restorative Dent. 1998 Feb;18(1):8-23
pubmed: 9558553
J Oral Maxillofac Surg. 1996 Apr;54(4):420-32; discussion 432-3
pubmed: 8600258
J Microsc. 2002 Apr;206(Pt 1):33-40
pubmed: 12000561
Swed Dent J. 1994;18(1-2):29-34
pubmed: 8052949
Biomaterials. 2005 Jun;26(17):3503-9
pubmed: 15621240
Clin Oral Implants Res. 2015 May;26(5):501-6
pubmed: 24954120
Science. 1991 Nov 22;254(5035):1178-81
pubmed: 1957169
Injury. 2011 Jun;42(6):609-13
pubmed: 21489529
Opt Express. 2013 Aug 12;21(16):19324-38
pubmed: 23938849
Tissue Eng Part C Methods. 2014 Apr;20(4):308-16
pubmed: 23879738
Int J Mol Sci. 2017 Feb 10;18(2):
pubmed: 28208578
Int J Periodontics Restorative Dent. 1996 Jun;16(3):220-9
pubmed: 9084308
Int J Periodontics Restorative Dent. 1994 Dec;14(6):496-511
pubmed: 7751115
Photomed Laser Surg. 2008 Aug;26(4):371-7
pubmed: 18647094
Photomed Laser Surg. 2004 Jun;22(3):249-53
pubmed: 15315733
Lasers Med Sci. 2012 Mar;27(2):423-30
pubmed: 21597948
J Clin Laser Med Surg. 2003 Oct;21(5):271-7
pubmed: 14651794
Arq Bras Cir Dig. 2012 Jan-Mar;25(1):49-51
pubmed: 22569979
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Jun;97(6):693-700
pubmed: 15184850
J Biomed Opt. 2017 Dec;22(12):1-30
pubmed: 29260539
Quant Imaging Med Surg. 2019 May;9(5):782-798
pubmed: 31281774
J Photochem Photobiol B. 2003 May-Jun;70(2):81-9
pubmed: 12849698
J Synchrotron Radiat. 2011 Jul;18(Pt 4):617-29
pubmed: 21685680
Lasers Med Sci. 2002;17(3):216-20
pubmed: 12181636
J Craniofac Surg. 2002 Jan;13(1):111-21; discussion 122-3
pubmed: 11887007
Photochem Photobiol. 2004 Sep-Oct;80(2):366-72
pubmed: 15362946
Photomed Laser Surg. 2005 Feb;23(1):27-31
pubmed: 15782028
AIMS Biophys. 2017;4(3):337-361
pubmed: 28748217