Decreased expression of a phagocytic receptor Siglec-1 on alveolar macrophages in chronic obstructive pulmonary disease.
Aged
Cells, Cultured
Female
Flow Cytometry
/ methods
Gene Expression
Humans
Macrophages, Alveolar
/ metabolism
Male
Middle Aged
Phagocytes
/ metabolism
Phagocytosis
/ physiology
Pulmonary Disease, Chronic Obstructive
/ genetics
Respiratory Function Tests
/ methods
Sialic Acid Binding Ig-like Lectin 1
/ biosynthesis
Alveolar macrophage
Chronic obstructive pulmonary disease
Phagocytosis
Siglec-1
Journal
Respiratory research
ISSN: 1465-993X
Titre abrégé: Respir Res
Pays: England
ID NLM: 101090633
Informations de publication
Date de publication:
28 Jan 2020
28 Jan 2020
Historique:
received:
17
10
2019
accepted:
16
01
2020
entrez:
30
1
2020
pubmed:
30
1
2020
medline:
21
10
2020
Statut:
epublish
Résumé
Alveolar macrophages are professional phagocytes that remove microbial pathogens inhaled into the lung. The phagocytic ability is compromised in chronic obstructive pulmonary disease (COPD). However, the molecular mechanisms underlying this defect in phagocytosis are not clearly defined. Cell suspensions were collected from lung tissues of patients undergoing lung resection. Alveolar macrophages were detected as FSC The flow-cytometry analyses revealed that FcγRI, CD11b, MSR-1 and Siglec-1, but not CD36, were expressed on human alveolar macrophages. Among these receptors, Siglec-1 expression was significantly decreased on alveolar macrophages in COPD ex-smokers (n = 11), compared to control never-smokers (n = 11) or control ex-smokers (n = 9). The Siglec-1 expression on alveolar macrophages was significantly correlated with lung function (forced expiratory volume in 1 s) and with the severity of emphysema. Treatment of human alveolar macrophages with an anti-Siglec1 blocking antibody decreased phagocytosis of non-typeable Haemophilus influenzae (NTHi). Our findings demonstrated reduced expression of Siglec-1 on alveolar macrophages in COPD, which is involved in engulfment of NTHi.
Sections du résumé
BACKGROUND
BACKGROUND
Alveolar macrophages are professional phagocytes that remove microbial pathogens inhaled into the lung. The phagocytic ability is compromised in chronic obstructive pulmonary disease (COPD). However, the molecular mechanisms underlying this defect in phagocytosis are not clearly defined.
MATERIALS AND METHODS
METHODS
Cell suspensions were collected from lung tissues of patients undergoing lung resection. Alveolar macrophages were detected as FSC
RESULTS
RESULTS
The flow-cytometry analyses revealed that FcγRI, CD11b, MSR-1 and Siglec-1, but not CD36, were expressed on human alveolar macrophages. Among these receptors, Siglec-1 expression was significantly decreased on alveolar macrophages in COPD ex-smokers (n = 11), compared to control never-smokers (n = 11) or control ex-smokers (n = 9). The Siglec-1 expression on alveolar macrophages was significantly correlated with lung function (forced expiratory volume in 1 s) and with the severity of emphysema. Treatment of human alveolar macrophages with an anti-Siglec1 blocking antibody decreased phagocytosis of non-typeable Haemophilus influenzae (NTHi).
CONCLUSION
CONCLUSIONS
Our findings demonstrated reduced expression of Siglec-1 on alveolar macrophages in COPD, which is involved in engulfment of NTHi.
Identifiants
pubmed: 31992280
doi: 10.1186/s12931-020-1297-2
pii: 10.1186/s12931-020-1297-2
pmc: PMC6986024
doi:
Substances chimiques
SIGLEC1 protein, human
0
Sialic Acid Binding Ig-like Lectin 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
30Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP17H04180
Organisme : Japan Society for the Promotion of Science
ID : JP16K15453
Organisme : Japan Society for the Promotion of Science
ID : JP26293195
Organisme : Japan Society for the Promotion of Science
ID : JP16H05307
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