Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
2019-nCoV
RT-PCR
Wuhan
diagnostics
laboratory
novel coronavirus
outbreak
testing
Journal
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
ISSN: 1560-7917
Titre abrégé: Euro Surveill
Pays: Sweden
ID NLM: 100887452
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
entrez:
30
1
2020
pubmed:
30
1
2020
medline:
20
2
2020
Statut:
ppublish
Résumé
The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project. The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
Sections du résumé
BACKGROUND
The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.
AIM
We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.
METHODS
Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
RESULTS
The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project.
CONCLUSION
The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
Identifiants
pubmed: 31992387
doi: 10.2807/1560-7917.ES.2020.25.3.2000045
pmc: PMC6988269
doi:
Substances chimiques
COVID-19 Vaccines
0
Covid-19 aAPC vaccine
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
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