In vitro induction of quiescence in isolated primary human myoblasts.
Cell cycle inhibition
Isolation
Primary human myoblasts
Quiescence
Journal
Cytotechnology
ISSN: 0920-9069
Titre abrégé: Cytotechnology
Pays: United States
ID NLM: 8807027
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
30
08
2019
accepted:
30
12
2019
pubmed:
30
1
2020
medline:
30
1
2020
entrez:
30
1
2020
Statut:
ppublish
Résumé
Adult skeletal muscle stem cells, satellite cells, remain in an inactive or quiescent state in vivo under physiological conditions. Progression through the cell cycle, including activation of quiescent cells, is a tightly regulated process. Studies employing in vitro culture of satellite cells, primary human myoblasts (PHMs), necessitate isolation myoblasts from muscle biopsies. Further studies utilizing these cells should endeavour to represent their native in vivo characteristics as closely as possible, also considering variability between individual donors. This study demonstrates the approach of utilizing KnockOut™ Serum Replacement (KOSR)-supplemented culture media as a quiescence-induction media for 10 days in PHMs isolated and expanded from three different donors. Cell cycle analysis demonstrated that treatment resulted in an increase in G
Identifiants
pubmed: 31993891
doi: 10.1007/s10616-019-00365-8
pii: 10.1007/s10616-019-00365-8
pmc: PMC7192999
doi:
Types de publication
Journal Article
Langues
eng
Pagination
189-202Subventions
Organisme : National Research Foundation: South African Research Chairs Initiative (SARChI)
ID : SARCI150212114075
Références
Sports Med. 2008;38(11):947-69
pubmed: 18937524
Methods Enzymol. 1979;58:248-62
pubmed: 423765
Cell Stem Cell. 2008 Jan 10;2(1):22-31
pubmed: 18371418
Cell. 2004 Mar 19;116(6):769-78
pubmed: 15035980
Methods Mol Biol. 2011;761:75-83
pubmed: 21755442
Dev Biol. 2007 Dec 1;312(1):13-28
pubmed: 17961534
Biotechnol J. 2018 Feb;13(2):
pubmed: 29334181
Biopreserv Biobank. 2019 Aug;17(4):342-351
pubmed: 31009253
Cell. 2009 May 1;137(3):413-31
pubmed: 19410540
Stem Cells. 2015 Mar;33(3):951-61
pubmed: 25447026
Nature. 2008 Nov 27;456(7221):502-6
pubmed: 18806774
Genes Dev. 1996 May 15;10(10):1173-83
pubmed: 8675005
J Orthop Res. 2007 Aug;25(8):1029-41
pubmed: 17469183
Stem Cell Res. 2018 Jul;30:122-129
pubmed: 29879622
Bio Protoc. 2017 Nov 5;7(21):
pubmed: 29152538
In Vitro Cell Dev Biol Anim. 1996 Feb;32(2):90-9
pubmed: 8907122
ALTEX. 2010;27(1):53-62
pubmed: 20390239
J Cell Sci. 2012 Mar 1;125(Pt 5):1309-17
pubmed: 22349695
J Biophys Biochem Cytol. 1961 Feb;9:493-5
pubmed: 13768451
Dev Biol. 1996 Apr 10;175(1):84-94
pubmed: 8608871
Yakugaku Zasshi. 2011;131(9):1329-32
pubmed: 21881307
J Cell Biol. 1999 Feb 22;144(4):631-43
pubmed: 10037786
PLoS One. 2013 May 23;8(5):e64067
pubmed: 23717533
Bio Protoc. 2017 May 5;7(9):
pubmed: 28730161
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6792-6
pubmed: 3045824
Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16552-7
pubmed: 17940048
Cell Stem Cell. 2012 Jul 6;11(1):118-26
pubmed: 22770245
PLoS One. 2015 Oct 16;10(10):e0140585
pubmed: 26473951
Cell Transplant. 2012;21(1):153-73
pubmed: 21669036
J Cell Biol. 1994 Jun;125(6):1275-87
pubmed: 8207057
J Histochem Cytochem. 2010 Nov;58(11):941-55
pubmed: 20644208
Cell Mol Life Sci. 2001 Apr;58(4):571-9
pubmed: 11361092
Cell Rep. 2013 Jul 11;4(1):189-204
pubmed: 23810552
Int J Mol Sci. 2019 Oct 24;20(21):
pubmed: 31652937
J Vis Exp. 2015 Jan 12;(95):52049
pubmed: 25650991
J Biol Chem. 1990 Jun 5;265(16):9015-21
pubmed: 2188970
Genes Dev. 1990 Sep;4(9):1454-61
pubmed: 2253873