Closed Bipolar Electrode Array for On-Chip Analysis of Cellular Respiration by Cell Aggregates.

Cell aggregates have attracted much attention owing to their potential applications in tissue engineering and drug screening. To evaluate cellular respiration of individual cell aggregates in these applications, noninvasive and on-chip high-throughput analytical tools are necessary. Electrochemical methods for detecting oxygen concentrations are useful because of their noninvasiveness. However, these conventional methods may be unsuitable for high-throughput detection because it is difficult to prepare many electrodes on a small chip owing to the limitation of area for connecting electrodes. Alternatively, a bipolar electrode (BPE) system offers clear advantages. In this system, electrochemical reactions are induced at both ends of a BPE without complex wiring. In this study, we present a BPE array for detecting the respiratory activity of cell aggregates. Oxygen concentrations near cell aggregates at cathodic poles of BPEs were converted to electrochemiluminescence (ECL) signals of [Ru(bpy)