Association between cytokines and two circulating micro-RNAs and development of premature ventricular contractions-induced cardiomyopathy.
Cardiomyopathiy
Cytokines
Inflammation
MicroRNAs
Peripheral blood-mononuclear cells
Journal
Iranian journal of basic medical sciences
ISSN: 2008-3866
Titre abrégé: Iran J Basic Med Sci
Pays: Iran
ID NLM: 101517966
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
entrez:
31
1
2020
pubmed:
31
1
2020
medline:
31
1
2020
Statut:
ppublish
Résumé
Recent progress in understanding the pathogenesis of premature ventricular contraction (PVC)-induced cardiomyopathy (PIC) has suggested a key role for inflammation. The aim of this study was to evaluate the expression of messenger RNAs (mRNAs) and the protein production of interleukin-6 (IL-6), IL-10, tumor necrosis factor alpha (TNF-α) and interferon-γ (IFN-γ) and two circulating micro-RNAs related to inflammation and cardiovascular disease; miR-155 and miR-146. The study population was comprised 25 patients with PIC and 25 patients with normal left ventricular ejection fraction despite frequent PVCs. TNF-α, IL-6, IL10, and IFN-γ levels were evaluated in peripheral blood mononuclear cells (PBMCs) by flow cytometry and their mRNAs were assessed by real time PCR. We analyzed circulating levels of these cytokines by enzyme linked immunosorbent assay (ELISA). Two circulating micro-RNAs, miR-155 and miR-146a, were also investigated. The flow cytometry findings showed that the median fluorescence intensity (MFI) of antibodies reacted with the IL-6 and TNF-α were higher in PIC group than the control group ( In our patients with PIC, there was an elevation in the expression levels of IL-6 and TNF-α in PBMCs. This finding may provide further insights into the inflammatory pathways involved in PIC.
Identifiants
pubmed: 31998452
doi: 10.22038/ijbms.2019.36362.8662
pmc: PMC6885395
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1125-1131Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest.
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