Spatiotemporal control of phosphatidic acid signaling with optogenetic, engineered phospholipase Ds.
Adaptor Proteins, Signal Transducing
/ metabolism
Bacterial Proteins
/ genetics
Biosensing Techniques
Cell Membrane
/ enzymology
HEK293 Cells
Hippo Signaling Pathway
Humans
Hydrolysis
Intracellular Membranes
/ enzymology
Optogenetics
Phosphatidic Acids
/ metabolism
Phosphatidylcholines
/ metabolism
Phospholipase D
/ genetics
Protein Engineering
Protein Serine-Threonine Kinases
/ metabolism
Second Messenger Systems
Substrate Specificity
Time Factors
Transcription Factors
/ metabolism
YAP-Signaling Proteins
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
02 03 2020
02 03 2020
Historique:
received:
24
07
2019
revised:
09
11
2019
accepted:
17
12
2019
entrez:
31
1
2020
pubmed:
31
1
2020
medline:
21
10
2020
Statut:
ppublish
Résumé
Phosphatidic acid (PA) is both a central phospholipid biosynthetic intermediate and a multifunctional lipid second messenger produced at several discrete subcellular locations. Organelle-specific PA pools are believed to play distinct physiological roles, but tools with high spatiotemporal control are lacking for unraveling these pleiotropic functions. Here, we present an approach to precisely generate PA on demand on specific organelle membranes. We exploited a microbial phospholipase D (PLD), which produces PA by phosphatidylcholine hydrolysis, and the CRY2-CIBN light-mediated heterodimerization system to create an optogenetic PLD (optoPLD). Directed evolution of PLD using yeast membrane display and IMPACT, a chemoenzymatic method for visualizing cellular PLD activity, yielded a panel of optoPLDs whose range of catalytic activities enables mimicry of endogenous, physiological PLD signaling. Finally, we applied optoPLD to elucidate that plasma membrane, but not intracellular, pools of PA can attenuate the oncogenic Hippo signaling pathway. OptoPLD represents a powerful and precise approach for revealing spatiotemporally defined physiological functions of PA.
Identifiants
pubmed: 31999306
pii: 133675
doi: 10.1083/jcb.201907013
pmc: PMC7054994
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Bacterial Proteins
0
Phosphatidic Acids
0
Phosphatidylcholines
0
Transcription Factors
0
YAP-Signaling Proteins
0
YAP1 protein, human
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
Phospholipase D
EC 3.1.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Tei and Baskin.
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