Expression and Cellular Distribution of P-Glycoprotein and Breast Cancer Resistance Protein in Amyotrophic Lateral Sclerosis Patients.


Journal

Journal of neuropathology and experimental neurology
ISSN: 1554-6578
Titre abrégé: J Neuropathol Exp Neurol
Pays: England
ID NLM: 2985192R

Informations de publication

Date de publication:
01 03 2020
Historique:
received: 15 06 2019
revised: 23 11 2019
accepted: 20 12 2019
pubmed: 31 1 2020
medline: 22 7 2020
entrez: 31 1 2020
Statut: ppublish

Résumé

For amyotrophic lateral sclerosis (ALS), achieving and maintaining effective drug levels in the brain is challenging due to the activity of ATP-binding cassette (ABC) transporters which efflux drugs that affect drug exposure and response in the brain. We investigated the expression and cellular distribution of the ABC transporters P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) using immunohistochemistry in spinal cord (SC), motor cortex, and cerebellum from a large cohort of genetically well characterized ALS patients (n = 25) and controls (n = 14). The ALS group included 17 sporadic (sALS) and 8 familial (fALS) patients. Strong P-gp expression was observed in endothelial cells in both control and ALS specimens. Immunohistochemical analysis showed higher P-gp expression in reactive astroglial cells in both gray (ventral horn) and white matter of the SC, as well as in the motor cortex of all ALS patients, as compared with controls. BCRP expression was higher in glia in the SC and in blood vessels and glia in the motor cortex of ALS patients, as compared with controls. P-gp and BCRP immunoreactivity did not differ between sALS and fALS cases. The upregulation of both ABC transporters in the brain may explain multidrug resistance in ALS patients and has implications for the use of both approved and experimental therapeutics.

Identifiants

pubmed: 31999342
pii: 5718003
doi: 10.1093/jnen/nlz142
pmc: PMC7036662
doi:

Substances chimiques

ABCG2 protein, human 0
ATP Binding Cassette Transporter, Subfamily B, Member 1 0
ATP Binding Cassette Transporter, Subfamily G, Member 2 0
Neoplasm Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

266-276

Informations de copyright

© 2019 American Association of Neuropathologists, Inc.

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Auteurs

Erwin A van Vliet (EA)

From the Amsterdam UMC, University of Amsterdam, Department of (Neuro)Pathology, Amsterdam Neuroscience.
Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam.

Anand M Iyer (AM)

From the Amsterdam UMC, University of Amsterdam, Department of (Neuro)Pathology, Amsterdam Neuroscience.

Lucia Mesarosova (L)

From the Amsterdam UMC, University of Amsterdam, Department of (Neuro)Pathology, Amsterdam Neuroscience.

Hilal Çolakoglu (H)

Division of Pharmacology, The Netherlands Cancer Institute (HÇ, OvT), Amsterdam, The Netherlands.

Jasper J Anink (JJ)

From the Amsterdam UMC, University of Amsterdam, Department of (Neuro)Pathology, Amsterdam Neuroscience.

Olaf van Tellingen (O)

Division of Pharmacology, The Netherlands Cancer Institute (HÇ, OvT), Amsterdam, The Netherlands.

Nicholas J Maragakis (NJ)

Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Jeremy Shefner (J)

Department of Neurology, Barrow Neurological Institute, Phoenix, Arizona.

Ton Bunt (T)

Izumi Biosciences, Inc., Lexington, Massachusetts.

Eleonora Aronica (E)

From the Amsterdam UMC, University of Amsterdam, Department of (Neuro)Pathology, Amsterdam Neuroscience.

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