Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death.

Animals Biomarkers, Tumor / analysis Calreticulin / analysis Flow Cytometry / methods Humans Immunogenic Cell Death Immunohistochemistry / methods Neoplasms / immunology Tumor Microenvironment
Calreticulin Cancer prognosis Flow cytometry Immunogenic cell death Immunohistochemistry

Journal

Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271

Informations de publication

Date de publication:
2020
Historique:
entrez: 1 2 2020
pubmed: 1 2 2020
medline: 19 12 2020
Statut: ppublish

Résumé

Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.

Identifiants

DOI: 10.1016/bs.mie.2019.05.025 PMID: 32000894
pubmed: 32000894
pii: S0076-6879(19)30184-3
doi: 10.1016/bs.mie.2019.05.025
pii:
doi:

Substances chimiques

Biomarkers, Tumor 0
Calreticulin 0

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15-25

Informations de copyright

© 2020 Elsevier Inc. All rights reserved.

Auteurs

Lenka Kasikova (L)

Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.

Iva Truxova (I)

Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.

Isabelle Cremer (I)

Team Inflammation, Complement and Cancer, INSERM, Centre de Recherche des Cordeliers, Paris, France; Sorbonne Université, Paris, France.

Catherine Sautes-Fridman (C)

Team Inflammation, Complement and Cancer, INSERM, Centre de Recherche des Cordeliers, Paris, France; Sorbonne Université, Paris, France.

Oliver Kepp (O)

Centre de Recherche des Cordeliers, Equipe labellisée par la Ligue contre le cancer, Université de Paris Descartes, Université Sorbonne Paris Cité, Université Paris Diderot, Sorbonne Université, INSERM, Paris, France; Metabolomics and Cell Biology Platforms, Institut Gustave Roussy, Villejuif, France; Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France.

Guido Kroemer (G)

Centre de Recherche des Cordeliers, Equipe labellisée par la Ligue contre le cancer, Université de Paris Descartes, Université Sorbonne Paris Cité, Université Paris Diderot, Sorbonne Université, INSERM, Paris, France; Metabolomics and Cell Biology Platforms, Institut Gustave Roussy, Villejuif, France; Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France; Suzhou Institute for Systems Biology, Chinese Academy of Sciences, Suzhou, China; Department of Women's and Children's Health, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden.

Radek Spisek (R)

Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic.

Jitka Fucikova (J)

Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic. Electronic address: fucikova@sotio.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lectines › Calréticuline › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Mort cellulaire immunogène › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Immunohistochimie › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Tumeurs › Side-by-side comparison of flow cytometry and...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Microenvironnement cellulaire › Microenvironnement tumoral › Side-by-side comparison of flow cytometry and...