Isolation of mononuclear phagocytes from the mouse gut.

Animals Antigens, CD / analysis Cell Separation / methods Dendritic Cells / cytology Female Flow Cytometry / methods Gastrointestinal Microbiome Intestines / cytology Macrophages / cytology Male Mice Mice, Inbred C57BL Monocytes / cytology Mononuclear Phagocyte System / cytology Myeloid Cells / immunology Phagocytes / cytology Staining and Labeling / methods
Dendritic cells Heterogeneity Macrophages Mucosal immunity Myeloid

Journal

Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271

Informations de publication

Date de publication:
2020
Historique:
entrez: 1 2 2020
pubmed: 1 2 2020
medline: 19 12 2020
Statut: ppublish

Résumé

The intestinal tract is home to trillions of microbes that make up the gut microbiota and is a major source of environmental antigens that can be derived from food, commensal microorganisms, and potential pathogens. Amidst this complex environment, myeloid cells, including macrophages (MPs) and dendritic cells (DCs), are key immunological sentinels that locally maintain both tissue and immune homeostasis. Recent research has revealed substantial functional and developmental heterogeneity within the intestinal DC and MP compartments, with evidence pointing to their regulation by the microbiota. DCs are classically divided into three subsets based on their CD103 and CD11b expression: CD103

Identifiants

DOI: 10.1016/bs.mie.2019.10.004 PMID: 32000915
pubmed: 32000915
pii: S0076-6879(19)30406-9
doi: 10.1016/bs.mie.2019.10.004
pii:
doi:

Substances chimiques

Antigens, CD 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

67-90

Informations de copyright

© 2020 Elsevier Inc. All rights reserved.

Auteurs

Pailin Chiaranunt (P)

Department of Immunology, University of Toronto, Toronto, ON, Canada.

Kyle Burrows (K)

Department of Immunology, University of Toronto, Toronto, ON, Canada.

Louis Ngai (L)

Department of Immunology, University of Toronto, Toronto, ON, Canada.

Arthur Mortha (A)

Department of Immunology, University of Toronto, Toronto, ON, Canada. Electronic address: arthur.mortha@utoronto.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes CD › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Séparation cellulaire › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système phagocytaire mononucléé › Cellules dendritiques › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Système digestif › Tube digestif › Intestins › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Monocytes › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système phagocytaire mononucléé › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Cellules › Cellules myéloïdes › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Isolation of mononuclear phagocytes from the mouse gut.
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Isolation of mononuclear phagocytes from the mouse gut.