Early biological responses in ocular tissue after SMILE and LASIK surgery.
Aged
Aged, 80 and over
Blotting, Western
Chromatography, Liquid
Collagen
/ metabolism
Corneal Stroma
/ metabolism
Corneal Surgery, Laser
Crystallins
/ metabolism
Eye Proteins
/ metabolism
Humans
Keratomileusis, Laser In Situ
/ methods
Male
Metabolome
Middle Aged
Prospective Studies
Proteoglycans
/ metabolism
Proteome
/ metabolism
Tandem Mass Spectrometry
Biological response
LASIK
LC-MS/MS
Ocular tissue
Protein profile
Refractive surgery
SMILE
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
02
04
2019
revised:
09
12
2019
accepted:
14
01
2020
pubmed:
1
2
2020
medline:
24
10
2020
entrez:
1
2
2020
Statut:
ppublish
Résumé
We studied the early protein profile in the ocular tissue extracted after LASIK and SMILE surgery. SMILE and LASIK was performed in contralateral eyes and stromal tissue samples were collected from 10 eyes of 5 donors. The stromal tissue samples were analyzed using label free quantification approach and ITRAQ labelling approach in LC-MS/MS. Combined functional analysis revealed many differentially expressed proteins which were involved in important biological processes. About 117 unique differentially expressed proteins were identified using two different proteomic approaches. Collagens, proteoglycans, corneal crystallins were enriched and showed differential expression in SMILE and LASIK as compared to the non-surgical control. Apart from these, 14-3-3 class of proteins, Lysozyme (LYZ), Macrophage Migratory Inhibitory Factor protein (MIF), Pigment Epithelial Derived Factor (PEDF) were differentially expressed when compared between LASIK and SMILE. Peroxiredoxin 1 (PRDX1) expression was found to be reduced in LASIK as compared to SMILE. The expression of Lysozyme C and Macrophage Migratory Inhibitory Factor inflammatory response was found to be less in SMILE as compared to LASIK. Western blot validation of specific markers such as Collagen IV (COL4), Keratocan (KERA), Lumican (LUM), Aldehyde dehydrogenase 3 A1 (ALDH3A1), Lysozyme C (LYZC) confirmed the differences in the protein levels observed in SMILE and LASIK operated tissues as compared to non-surgical controls. In conclusion, this study revealed the early molecular changes occurring in the cornea resulting from these two surgical procedures which may have implications on managing post-operative complications.
Identifiants
pubmed: 32001250
pii: S0014-4835(19)30248-9
doi: 10.1016/j.exer.2020.107936
pii:
doi:
Substances chimiques
Crystallins
0
Eye Proteins
0
Proteoglycans
0
Proteome
0
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107936Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest P. Khamar, None; T. Nishtala, None; R. Shetty, None; P. Trailokyanath, None; K. Shetty, None; N. Pahuja, None; V. Deshpande, None; A. Ghosh, None.