Quantitative evaluation of the impact of artificial cell adhesion via DNA hybridization on E-cadherin-mediated cell adhesion.
Journal
APL bioengineering
ISSN: 2473-2877
Titre abrégé: APL Bioeng
Pays: United States
ID NLM: 101726398
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
21
08
2019
accepted:
12
12
2019
entrez:
1
2
2020
pubmed:
1
2
2020
medline:
1
2
2020
Statut:
epublish
Résumé
Programmable cell adhesion with DNA hybridization is a promising approach for fabricating various tissue architectures without sophisticated instrumentation. However, little is known about how this artificial interaction influences the binding of cell adhesion proteins, E-cadherin. In this work, we designed a planar and fluid lipid membrane displaying E-cadherin and/or single-strand DNA with well-defined densities. Visualization of cells on membranes by fluorescence and interference microscopy revealed cell adhesion to be a two-step process: artificial adhesion by DNA hybridization within a few minutes followed by biological adhesion via cadherin-cadherin binding within hours. Furthermore, we discovered that DNA hybridization can substantially facilitate E-cadherin-mediated cell adhesion. The promotive effect is probably due to the enforced binding between E-cadherin molecules in geometrical confinement between two membranes. Our
Identifiants
pubmed: 32002498
doi: 10.1063/1.5123749
pii: 1.5123749
pmc: PMC6984976
doi:
Types de publication
Journal Article
Langues
eng
Pagination
016103Informations de copyright
© Author(s).
Références
Annu Rev Biophys Biomol Struct. 2004;33:415-40
pubmed: 15139820
Structure. 2011 Feb 9;19(2):244-56
pubmed: 21300292
Sci Rep. 2013 Dec 10;3:3462
pubmed: 24322507
Nat Biotechnol. 2016 Mar;34(3):312-9
pubmed: 26878319
Biophys J. 2009 Nov 4;97(9):2630-9
pubmed: 19883607
Biochemistry. 2008 May 13;47(19):5336-53
pubmed: 18422348
Nature. 2013 Jan 17;493(7432):318-26
pubmed: 23325214
J Biol Chem. 2000 Dec 29;275(52):41227-33
pubmed: 10969083
Cell. 1996 Feb 9;84(3):345-57
pubmed: 8608588
Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4606-10
pubmed: 19273855
Biochim Biophys Acta Biomembr. 2017 Sep;1859(9 Pt A):1483-1492
pubmed: 27998689
Nature. 2011 Jul 27;475(7357):510-3
pubmed: 21796210
J Am Chem Soc. 2012 Jan 18;134(2):765-8
pubmed: 22176556
Cell Stem Cell. 2015 May 7;16(5):556-65
pubmed: 25891906
Curr Opin Cell Biol. 2011 Oct;23(5):523-30
pubmed: 21890337
Dev Biol. 2003 Jan 15;253(2):309-23
pubmed: 12645933
Nat Biotechnol. 2014 Aug;32(8):773-85
pubmed: 25093879
J Phys Chem B. 2013 May 2;117(17):5002-8
pubmed: 23586470
Biophys J. 1988 Jun;53(6):963-70
pubmed: 3395663
Science. 2002 May 17;296(5571):1308-13
pubmed: 11964443
Nat Cell Biol. 2015 May;17(5):533-9
pubmed: 25925582
Nature. 2014 Dec 18;516(7531):400-4
pubmed: 25363776
Chemphyschem. 2009 Nov 9;10(16):2752-68
pubmed: 19816893
J Phys Chem B. 2013 Apr 18;117(15):4081-8
pubmed: 23531225
Trends Cell Biol. 2012 Jun;22(6):299-310
pubmed: 22555008
Nat Rev Mol Cell Biol. 2010 Jul;11(7):502-14
pubmed: 20571587
J Cell Sci. 1998 Feb;111 ( Pt 3):347-57
pubmed: 9427683
Cell. 2009 Nov 25;139(5):871-90
pubmed: 19945376
Cell Adh Migr. 2012 Jan-Feb;6(1):59-70
pubmed: 22647941
J Cell Biol. 2003 Nov 24;163(4):847-57
pubmed: 14623871
Biomaterials. 2015 Apr;48:119-28
pubmed: 25701037
Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):10932-7
pubmed: 26290581
PLoS One. 2013;8(2):e54749
pubmed: 23424619
Nature. 2011 Feb 3;470(7332):105-9
pubmed: 21151107
Biomaterials. 2010 Mar;31(8):2229-35
pubmed: 20004971