Injectable Cardiac Cell Microdroplets for Tissue Regeneration.
cell delivery
droplets
hydrogels
microfluidics
microscale
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
26
08
2019
revised:
01
01
2020
pubmed:
1
2
2020
medline:
8
5
2021
entrez:
1
2
2020
Statut:
ppublish
Résumé
One of the strategies for heart regeneration includes cell delivery to the defected heart. However, most of the injected cells do not form quick cell-cell or cell-matrix interactions, therefore, their ability to engraft at the desired site and improve heart function is poor. Here, the use of a microfluidic system is reported for generating personalized hydrogel-based cellular microdroplets for cardiac cell delivery. To evaluate the system's limitations, a mathematical model of oxygen diffusion and consumption within the droplet is developed. Following, the microfluidic system's parameters are optimized and cardiac cells from neonatal rats or induced pluripotent stem cells are encapsulated. The morphology and cardiac specific markers are assessed and cell function within the droplets is analyzed. Finally, the cellular droplets are injected to mouse gastrocnemius muscle to validate cell retention, survival, and maturation within the host tissue. These results demonstrate the potential of this approach to generate personalized cellular microtissues, which can be injected to distinct regions in the body for treating damaged tissues.
Identifiants
pubmed: 32003928
doi: 10.1002/smll.201904806
pmc: PMC7113023
mid: EMS86124
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1904806Subventions
Organisme : European Research Council
ID : 637943
Pays : International
Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Références
Adv Mater. 2019 Jan;31(1):e1803895
pubmed: 30406960
Interface Focus. 2014 Apr 6;4(2):20130038
pubmed: 24748951
Nat Commun. 2018 Dec 18;9(1):5419
pubmed: 30560926
J Mol Cell Cardiol. 2006 Nov;41(5):876-84
pubmed: 16973174
J Tissue Eng Regen Med. 2013 Jun;7(6):421-33
pubmed: 22318999
J Biomed Mater Res A. 2008 Dec 15;87(4):1113-22
pubmed: 18837425
Curr Opin Biotechnol. 2015 Dec;35:86-93
pubmed: 26051090
Biotechnol Bioeng. 2003 May 20;82(4):403-14
pubmed: 12632397
Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16568-73
pubmed: 19805339
J Gastrointest Surg. 2009 Jun;13(6):1138-46
pubmed: 19291335
Small. 2016 May;12(20):2720-30
pubmed: 27038291
Curr Stem Cell Rep. 2016 Sep;2(3):207-220
pubmed: 28868235
Biotechnol Bioeng. 2014 Mar;111(3):618-31
pubmed: 24166004
J R Soc Interface. 2018 Jan;15(138):
pubmed: 29343633
Acta Biomater. 2015 Jan;11:3-16
pubmed: 25266503
J Mater Chem B. 2013 Oct 14;1(38):5128-5136
pubmed: 32261104
Semin Cell Dev Biol. 2017 Nov;71:75-83
pubmed: 28754442
Biomaterials. 2011 Feb;32(6):1477-83
pubmed: 21095000
Adv Healthc Mater. 2018 Apr;7(8):e1700939
pubmed: 29316363
Head Neck. 2009 May;31(5):655-63
pubmed: 19260110
J Mater Chem B. 2016 May 28;4(20):3515-3525
pubmed: 32263385
PLoS One. 2017 Mar 7;12(3):e0173206
pubmed: 28267799
J Am Coll Cardiol. 2007 Nov 27;50(22):2173-95
pubmed: 18036459
J Healthc Eng. 2011 Dec;2(4):427-446
pubmed: 22180835
Cells Tissues Organs. 2013;197(5):333-43
pubmed: 23571151
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):E1848-57
pubmed: 22645348
Tissue Eng. 2006 Oct;12(10):2843-52
pubmed: 17518653