Ultra-fast Cycling for Multiplexed Cellular Fluorescence Imaging.

bioorthogonal chemistry cancer click chemistry fluorescent probes tetrazines

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
20 04 2020
Historique:
received: 28 11 2019
revised: 08 01 2020
pubmed: 1 2 2020
medline: 16 3 2021
entrez: 1 2 2020
Statut: ppublish

Résumé

Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Herein, we describe an ultra-fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz)/trans-cyclooctene (TCO)-mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster (t

Identifiants

pubmed: 32004403
doi: 10.1002/anie.201915153
doi:

Substances chimiques

Cyclooctanes 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

6839-6846

Subventions

Organisme : NCI NIH HHS
ID : R01CA206890
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA069246
Pays : United States
Organisme : NCI NIH HHS
ID : T32CA079443
Pays : United States
Organisme : NCI NIH HHS
ID : UH3CA202637
Pays : United States
Organisme : NCI NIH HHS
ID : P01CA069246
Pays : United States

Informations de copyright

© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Auteurs

Jina Ko (J)

Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.

Juhyun Oh (J)

Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.

Maaz S Ahmed (MS)

Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.

Jonathan C T Carlson (JCT)

Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.
Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Ralph Weissleder (R)

Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.
Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.

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