A novel Xist RNA-mediated chromosome inactivation model using a mouse artificial chromosome.
Microcell-mediated chromosome transfer
Mouse artificial chromosome
X Chromosome inactivation
Xist (X-inactive specific transcript)
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
30
10
2019
accepted:
26
01
2020
pubmed:
2
2
2020
medline:
15
12
2020
entrez:
2
2
2020
Statut:
ppublish
Résumé
To develop a mouse artificial chromosome (MAC) carrying the mouse Xist gene (X-inactive specific transcript; Xist-MAC) as a systematic in vitro approach for investigating Xist RNA-mediated chromosome inactivation. Ectopic expression of the Xist gene in CHO cells led to the accumulation of Xist RNA in cis on the MAC. In addition, the introduction of Xist-MAC to embryonic stem cells from male mice via microcell-mediated chromosome transfer resulted in the accumulation of Xist RNA in cis on the MAC. Chromosomal inactivation was observed in the differentiated state. Moreover, this phenomenon was accompanied by the epigenetic modification of H3K27 trimethylation. We successfully generated a novel chromosome inactivation model, Xist-MAC, which will provide a valuable tool for the screening and functional analysis of X chromosome inactivation-related genes and proteins.
Identifiants
pubmed: 32006350
doi: 10.1007/s10529-020-02826-z
pii: 10.1007/s10529-020-02826-z
doi:
Substances chimiques
Histones
0
RNA, Long Noncoding
0
XIST non-coding RNA
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
697-705Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP17J03813
Organisme : Japan Society for the Promotion of Science
ID : JP25640114