A systematic approach to quantitative Western blot analysis.
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
15 03 2020
15 03 2020
Historique:
received:
01
08
2019
revised:
16
12
2019
accepted:
27
01
2020
pubmed:
3
2
2020
medline:
13
1
2021
entrez:
3
2
2020
Statut:
ppublish
Résumé
Attaining true quantitative data from WB requires that all the players involved in the procedure are quality controlled including the user. Appropriate protein extraction method, electrophoresis, and transfer of proteins, immunodetection of blotted protein by antibodies, and the ultimate step of imaging and analyzing the data is nothing short of a symphony. Like with any other technology in life-sciences research, Western blotting can produce erroneous and irreproducible data. We provide a systematic approach to generate quantitative data from Western blot experiments that incorporates critical validation steps to identify and minimize sources of error and variability throughout the Western blot process.
Identifiants
pubmed: 32007473
pii: S0003-2697(19)30750-X
doi: 10.1016/j.ab.2020.113608
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
113608Informations de copyright
Copyright © 2020 LI-COR BIOSCIENCES, LINCOLN, NEBRASKA 68504. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest L. Pillai-Kastoori, A. Schutz-Geschwender, and Jeff A. Harford are employees of LI-COR Biosciences.