The African swine fever virus isolate Belgium 2018/1 shows high virulence in European wild boar.
African Swine Fever
/ pathology
African Swine Fever Virus
/ immunology
Animals
Animals, Wild
/ virology
Antibodies, Viral
/ blood
Belgium
Enzyme-Linked Immunosorbent Assay
/ veterinary
Genome, Viral
Real-Time Polymerase Chain Reaction
/ veterinary
Sus scrofa
/ virology
Swine
Swine Diseases
/ pathology
Virulence
/ physiology
African swine fever virus
Belgium
histopathology
viral distribution
virulence
wild boar
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
23
12
2019
accepted:
29
01
2020
pubmed:
6
2
2020
medline:
18
11
2020
entrez:
4
2
2020
Statut:
ppublish
Résumé
African swine fever (ASF) is one of the most important and complex viral diseases in domestic pigs and wild boar. Over the last decade, the disease has spread to several European and Asian countries and is now one of the major threats to profitable pig production worldwide. One of the more recently affected western countries is Belgium. To date, only wild boar are affected in a rather defined area in the Luxembourg region close to France, Luxembourg and Germany. While detailed sequence analyses were recently performed, biological characterization was still pending. Here, we report on the experimental inoculation of four sub-adult wild boar to further characterize the virus and its distribution in different tissues. After oronasal inoculation with the virus strain 'Belgium 2018/1', all animals developed an acute and severe disease course with typical pathomorphological and histopathological lesions. Organs and blood samples were positive in qPCR, haemadsorption test and antigen lateral flow devices (LFD). Virus and viral genome were also detected in genitals and accessory sex glands of two boars. There were no antibodies detectable in commercial antibody ELISAs, antibody LFDs and indirect immunoperoxidase tests. Thus, the genotype II ASF virus isolate 'Belgium 2018/1' showed a highly virulent phenotype in European wild boar similar to parental viruses like Armenia 2007 and other previously characterized ASFV strains. The study also provided a large set of well-characterized sample materials for test validation and assay harmonization.
Substances chimiques
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1654-1659Subventions
Organisme : Friedrich-Loeffler-Institut
Organisme : European Cooperation in Science and Technology
ID : 15116
Informations de copyright
© 2020 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.
Références
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