Angiogenesis-Related Genes in Endothelial Progenitor Cells May Be Involved in Sickle Cell Stroke.


Journal

Journal of the American Heart Association
ISSN: 2047-9980
Titre abrégé: J Am Heart Assoc
Pays: England
ID NLM: 101580524

Informations de publication

Date de publication:
04 02 2020
Historique:
entrez: 4 2 2020
pubmed: 6 2 2020
medline: 22 12 2020
Statut: ppublish

Résumé

Background The clinical aspects of sickle cell anemia (SCA) are heterogeneous, and different patients may present significantly different clinical evolutions. Almost all organs can be affected, particularly the central nervous system. Transient ischemic events, infarcts, and cerebral hemorrhage can be observed and affect ≈25% of the patients with SCA. Differences in the expression of molecules produced by endothelial cells may be associated with the clinical heterogeneity of patients affected by vascular diseases. In this study, we investigated the differential expression of genes involved in endothelial cell biology in SCA patients with and without stroke. Methods and Results Endothelial progenitor cells from 4 SCA patients with stroke and 6 SCA patients without stroke were evaluated through the polymerase chain reaction array technique. The analysis of gene expression profiling identified 29 differentially expressed genes. Eleven of these genes were upregulated, and most were associated with angiogenesis (55%), inflammatory response (18%), and coagulation (18%) pathways. Downregulated expression was observed in 18 genes, with the majority associated with angiogenesis (28%), apoptosis (28%), and cell adhesion (22%) pathways. Remarkable overexpression of the

Identifiants

pubmed: 32009522
doi: 10.1161/JAHA.119.014143
pmc: PMC7033889
doi:

Substances chimiques

Angiogenic Proteins 0
MMP1 protein, human EC 3.4.24.7
Matrix Metalloproteinase 1 EC 3.4.24.7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e014143

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Auteurs

Mirta T Ito (MT)

Center for Molecular Biology and Genetic Engineering University of Campinas-UNICAMP Campinas São Paulo Brazil.

Sueli M da Silva Costa (SM)

Center for Molecular Biology and Genetic Engineering University of Campinas-UNICAMP Campinas São Paulo Brazil.

Letícia C Baptista (LC)

Center for Molecular Biology and Genetic Engineering University of Campinas-UNICAMP Campinas São Paulo Brazil.

Gabriela Q Carvalho-Siqueira (GQ)

Center for Molecular Biology and Genetic Engineering University of Campinas-UNICAMP Campinas São Paulo Brazil.

Dulcinéia M Albuquerque (DM)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Vinicius M Rios (VM)

Center for Molecular Biology and Genetic Engineering University of Campinas-UNICAMP Campinas São Paulo Brazil.

Stephanie Ospina-Prieto (S)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Roberta C Saez (RC)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Karla P Vieira (KP)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Fernando Cendes (F)

Neuroimaging Laboratory Department of Neurology University of Campinas, UNICAMP Campinas São Paulo Brazil.

Margareth C Ozelo (MC)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Sara Teresinha O Saad (STO)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Fernando F Costa (FF)

Hematology and Hemotherapy Center University of Campinas-UNICAMP Campinas São Paulo Brazil.

Mônica B Melo (MB)

Center for Molecular Biology and Genetic Engineering University of Campinas-UNICAMP Campinas São Paulo Brazil.

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Classifications MeSH