Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.
DNA assembly
Gibson assembly-derived cloning
molecular cloning
plant gene expression
prokaryotic expression
standardized cloning
vector construction
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2019
2019
Historique:
received:
19
07
2019
accepted:
19
12
2019
entrez:
4
2
2020
pubmed:
6
2
2020
medline:
6
2
2020
Statut:
epublish
Résumé
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as "Nimble Cloning" uses the restriction enzyme,
Identifiants
pubmed: 32010678
doi: 10.3389/fbioe.2019.00460
pmc: PMC6974442
doi:
Types de publication
Journal Article
Langues
eng
Pagination
460Informations de copyright
Copyright © 2020 Yan, Zeng, Shen, Tuo, Li and Zhou.
Références
Nucleic Acids Res. 2017 Feb 28;45(4):2196-2209
pubmed: 28053117
PLoS One. 2011 Feb 18;6(2):e16765
pubmed: 21364738
PLoS One. 2013 Dec 20;8(12):e83043
pubmed: 24376629
Nat Protoc. 2014 Sep;9(9):2075-89
pubmed: 25101822
PLoS One. 2014 Sep 22;9(9):e107907
pubmed: 25243603
Methods Mol Biol. 2011;729:167-81
pubmed: 21365490
Nat Struct Mol Biol. 2016 Jul;23(7):640-6
pubmed: 27273516
Nucleic Acids Res. 2014 Jan;42(1):681-9
pubmed: 24078086
PLoS One. 2009;4(5):e5553
pubmed: 19436741
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4
pubmed: 4594039
PLoS One. 2014 Dec 31;9(12):e115318
pubmed: 25551825
PLoS One. 2016 Apr 13;11(4):e0153158
pubmed: 27073895
Nucleic Acids Res. 2019 Feb 20;47(3):e15
pubmed: 30462336
Nucleic Acids Res. 2010 May;38(8):2624-36
pubmed: 20385581
Biotechniques. 2007 Sep;43(3):354-9
pubmed: 17907578
Methods Mol Biol. 2018;1671:131-147
pubmed: 29170957
Plant J. 2014 Nov;80(3):553-67
pubmed: 25187041
Plant Mol Biol. 2000 Apr;42(6):819-32
pubmed: 10890530
J Mol Biol. 2006 Oct 6;362(5):1120-31
pubmed: 16949607
PLoS One. 2008;3(11):e3647
pubmed: 18985154
Front Plant Sci. 2016 Mar 01;7:246
pubmed: 26973687
Plant Cell Rep. 2008 Jun;27(6):1053-63
pubmed: 18317773
Nucleic Acids Res. 2014 Jan;42(1):e7
pubmed: 24153110
PLoS One. 2012;7(5):e38186
pubmed: 22675447
Nat Protoc. 2006;1(4):2019-25
pubmed: 17487191
Genome Res. 2000 Nov;10(11):1788-95
pubmed: 11076863
Nucleic Acids Res. 2018 Nov 2;46(19):e113
pubmed: 29986052
Virology. 2017 Oct;510:99-103
pubmed: 28715654
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
PLoS One. 2011;6(7):e21622
pubmed: 21750718
Nucleic Acids Res. 2017 Jun 20;45(11):e94
pubmed: 28334760
ACS Synth Biol. 2018 May 18;7(5):1477-1480
pubmed: 29715010
Biotechniques. 2012 Oct 02;53(5):311-14
pubmed: 23066669